LOCUS       sequence-127082-        5489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_475_S2-1-GATA_dCP
FEATURES             Location/Qualifiers
     source          1..5489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(7..798,799..867))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     630..649
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(868..972)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      999..1454
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1086..1105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1296..1317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1585..1633
                     /note="synthetic polyadenylation signal"
     misc_feature    1647..1738
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1687..1706
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    1752..1776
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2283..2307)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2498..2507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2504..4156
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2570..2588)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4197..4318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4208..4227)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4262..4281
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4566..4583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4737..5325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4817..4836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
       61 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      121 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      181 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
      241 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
      301 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
      361 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      421 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      481 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      541 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      601 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      661 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      721 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      781 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      841 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      901 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      961 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     1021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     1321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     1381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     1441 aacgcttaca atttgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     1501 gcgggcctct tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt
     1561 acgggaggta cttggagcgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
     1621 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     1681 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat
     1741 cgataggtac cacaagtttg tacaaaaaag caggctccga attcgccctt tgcacaattt
     1801 ctgtgaagtg acagccactg agaaatagtg tcaagtagct ctcgtccgct aatatcaatt
     1861 tagacgatat acatacggcc agatctggca cgcagtctgc attcccaatg atgcgggatg
     1921 gatttggatt gtggggcgga atctctaagt cggagtactg tcctccgagg cggagaatta
     1981 cttccgcgac ttggctccgt tttgttaaat atactatgtg tgtatgtgcg tagtacagcg
     2041 acttgtacgg agtacataaa catatgcata tctgcggttt agttgtcgct cggaggacag
     2101 tactccgttg ttcgtcgttt gaaaaatgtg tgtagttgtt ggggtaactg cgcacaattg
     2161 ccgggctaca gggtgctttg agtctttgtt tttggatttc gttttcgatg tttagctacg
     2221 aaagccaagc gttgtgctat tttcgaattt tgtattttat aacccgggca agggcgaatt
     2281 cgacccagct ttcttgtaca aagtggtaga tctgcgatcg agctcgcccg gggatcgagc
     2341 gcagcggtat aaaagggcgc ggggtggctg agagcatcag ttgtgaatga atgttcgagc
     2401 cgagcagacg tgccgctgcc ttcgttaata tcctttgaat aagccaactt tgaatcacaa
     2461 gacgcatacc aaacggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa
     2521 cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact
     2581 gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca
     2641 tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc
     2701 tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct
     2761 tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa
     2821 cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt
     2881 gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat
     2941 ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac
     3001 gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt
     3061 cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa
     3121 aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat
     3181 ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg
     3241 ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta
     3301 tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct
     3361 gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt
     3421 atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc
     3481 ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac
     3541 atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt
     3601 tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca
     3661 aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga
     3721 agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg
     3781 ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg
     3841 ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga
     3901 cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt
     3961 tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt
     4021 aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct
     4081 taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg
     4141 aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag acatgataag
     4201 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4261 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4321 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     4381 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct
     4441 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     4501 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
     4561 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4621 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4681 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4741 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4801 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4861 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4921 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4981 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5041 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5101 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5161 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5221 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5281 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5341 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5401 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5461 atctaaagta tatatgagta aacttggtc
//
