LOCUS       sequence-127097-        6113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_MC19_Trl-2xUAS_dCP
FEATURES             Location/Qualifiers
     source          1..6113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(22..813,814..882))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    987..1035
                     /note="synthetic polyadenylation signal"
     misc_feature    1049..1140
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1089..1108
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    1187..1211
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1288..1312)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1509..1518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1515..3167
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1581..1599)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3208..3329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3219..3238)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3273..3292
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3523..3880
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3523..3714
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3546..3566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3731..3866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3793..3812
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3911..4948
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             4927..4935
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4972..5020
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5176..5193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5347..5935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5427..5446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat
       61 ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg
      121 tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc
      181 ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc
      241 tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc
      301 gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc
      361 gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc
      421 acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa
      481 gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat
      541 gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta
      601 gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca
      661 tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag
      721 gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc
      781 agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc
      841 aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata
      901 ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa
      961 ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg
     1021 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     1081 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     1141 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaaacaa gtttgtacaa
     1201 aaaagcaggc ttcttgctct ctctgtttgc tctctcgccg ccgcccggag tactgtcctc
     1261 cgagtgtcgg agtactgtcc tccgaggacc cagctttctt gtacaaagtg gtatatcaag
     1321 atctgcgatc gagctcgccc ggggatcgag cgcagcggta taaaagggcg cggggtggct
     1381 gagagcatca gttgtgaatg aatgttcgag ccgagcagac gtgccgctgc cttcgttaat
     1441 atcctttgaa taagccaact ttgaatcaca agacgcatac caaacggcat tccggtactg
     1501 ttggtaaagc caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     1561 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     1621 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     1681 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     1741 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     1801 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     1861 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     1921 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     1981 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     2041 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     2101 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     2161 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     2221 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     2281 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     2341 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     2401 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     2461 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     2521 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     2581 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     2641 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     2701 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     2761 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     2821 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     2881 ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg
     2941 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     3001 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     3061 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     3121 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg
     3181 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     3241 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3301 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     3361 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     3421 aaatcgataa ggatccgttt gcgtattggg cgctcttccg ctgatctgcg cagcaccatg
     3481 gcctgaaata acctctgaaa gaggaacttg gttagctacc ttctgaggcg gaaagaacca
     3541 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3601 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3721 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3781 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     3841 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcgatt cttctgacac
     3901 tagcgccacc atgaagaagc ccgaactcac cgctaccagc gttgaaaaat ttctcatcga
     3961 gaagttcgac agtgtgagcg acctgatgca gttgtcggag ggcgaagaga gccgagcctt
     4021 cagcttcgat gtcggcggac gcggctatgt actgcgggtg aatagctgcg ctgatggctt
     4081 ctacaaagac cgctacgtgt accgccactt cgccagcgct gcactaccca tccccgaagt
     4141 gttggacatc ggcgagttca gcgagagcct gacatactgc atcagtagac gcgcccaagg
     4201 cgttactctc caagacctcc ccgaaacaga gctgcctgct gtgttacagc ctgtcgccga
     4261 agctatggat gctattgccg ccgccgacct cagtcaaacc agcggcttcg gcccattcgg
     4321 gccccaaggc atcggccagt acacaacctg gcgggatttc atttgcgcca ttgctgatcc
     4381 ccatgtctac cactggcaga ccgtgatgga cgacaccgtg tccgccagcg tagctcaagc
     4441 cctggacgaa ctgatgctgt gggccgaaga ctgtcccgag gtgcgccacc tcgtccatgc
     4501 cgacttcggc agcaacaacg tcctgaccga caacggccgc atcaccgccg taatcgactg
     4561 gtccgaagct atgttcgggg acagtcagta cgaggtggcc aacatcttct tctggcggcc
     4621 ctggctggct tgcatggagc agcagactcg ctacttcgag cgccggcatc ccgagctggc
     4681 cggcagccct cgtctgcgag cctacatgct gcgcatcggc ctggatcagc tctaccagag
     4741 cctcgtggac ggcaacttcg acgatgctgc ctgggctcaa ggccgctgcg atgccatcgt
     4801 ccgcagcggg gccggcaccg tcggtcgcac acaaatcgct cgccggagcg cagccgtatg
     4861 gaccgacggc tgcgtcgagg tgctggccga cagcggcaac cgccggccca gtacacgacc
     4921 gcgcgctaag gaggtaggtc gagtttaaac tctagaaccg gtcatggccg caataaaata
     4981 tctttatttt cattacatct gtgtgttggt tttttgtgtg ttcgaactag atgctgtcga
     5041 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     5101 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     5161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5761 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gca
//
