LOCUS       sequence-127099-        5144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_764_4xUAS_DOS_hkCP
FEATURES             Location/Qualifiers
     source          1..5144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    39..160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(50..69)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     104..123
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    395..419
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(522..546)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(560..577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(731..1319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(811..830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1490..2281,2282..2350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2113..2132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2351..2455)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2482..2937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2569..2588)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2779..2800
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3068..3116
                     /note="synthetic polyadenylation signal"
     misc_feature    3130..3221
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3170..3189
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             3490..5142
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3556..3574)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
       61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
      241 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
      301 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
      361 ttatcatgca actcgtagga caggtgccgg cagcacaagt ttgtacaaaa aagcaggctt
      421 ccggagtact gtcctccgag cggagtactg tcctccgagc ggaagcttgc atgcctgcag
      481 gtcggagtac tgtcctccga gcggagtact gtcctccgag gacccagctt tcttgtacaa
      541 agtggtgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1141 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2461 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     2521 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2581 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     2641 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     2701 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     2761 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     2821 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     2881 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc
     2941 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     3001 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag
     3061 cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     3121 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
     3181 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgatagg taccgagctc
     3241 ttacgcgtcc atgcatgcta tgggaattcc catcagacgt ctggctcgag atctgcaatc
     3301 ttgtaccaat agctaaaaac tcacatctcc agcgccatgc cgattttgtt ctctttcttt
     3361 ccggttgtca aaaggtacag atgcttggat tttatttctc cgaaatgaag aggttttctt
     3421 atcgaaaatg taataaatat gaacaattaa ctatcttttc cagtgcagtg catccttaac
     3481 cgcagaacaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg
     3541 gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
     3601 ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc
     3661 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
     3721 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
     3781 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
     3841 atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg
     3901 aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat
     3961 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
     4021 gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac
     4081 tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg
     4141 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
     4201 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
     4261 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt
     4321 caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa
     4381 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct
     4441 cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg
     4501 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     4561 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     4621 accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt
     4681 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     4741 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc
     4801 ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc
     4861 ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc
     4921 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     4981 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
     5041 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     5101 atcctcataa aggccaagaa gggcggaaag atcgccgtgt aatt
//
