LOCUS       sequence-127129-        8368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the human ciRS7/CDR1as circular RNA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Kramer MC, Liang D, Tatomer DC, Gold B, March ZM, Cherry S, Wilusz 
            JE
  TITLE     Combinatorial control of Drosophila circular RNA expression by 
            intronic repeats, hnRNPs, and SR proteins.
  JOURNAL   Genes Dev. 2015 Oct 8.
  PUBMED    26450910
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2015 Oct 8."
COMMENT     Sequence Label: pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon
FEATURES             Location/Qualifiers
     source          1..8368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3962..3979)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3968..4192
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4238..4666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4325..4344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4535..4556
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4675..4695)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4680..5009
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4860..4995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4922..4941
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5076..5870
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5130..5149)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5740..5759
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6044..6165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6081..6100)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6135..6154
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6214..6230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6214..6230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6227..6249)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6238..6254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6262..6268,6269..6286,6287..6292))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6307..6328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6445..6462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6616..7201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6696..6715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7372..8163,8164..8232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7995..8014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8233..8337)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattccat
      961 tgagaaatga ctgagttccg gtgctctcaa gtcattgatc tttgtcgact tttatttggt
     1021 ctctgtaata acgacttcaa aaacattaaa ttctgttgcg aagccagtaa gctacaaaaa
     1081 gaaaaaacaa gagagaatgc tatagtcgta tagtatagtt tcccgactat ctgataccca
     1141 ttacttatct agggggaatg cgaacccaaa attttatcag ttttctcgga tatcgataga
     1201 tattggggaa taaatttaaa taaataaatt ttgggcgggt ttagggcgtg gcaaaaagtt
     1261 ttttggcaaa tcgctagaaa tttacaagac ttataaaatt atgaaaaaat acaacaaaat
     1321 tttaaacacg tgggcgtgac agttttgggc ggttttaggg cgttagagta ggcgaggaca
     1381 gggttacatc gactaggctt tgatcctgat caagaatata tatactttat accgcttcct
     1441 tctacatgtt acctattttt caacgaatct agtatacctt tttactgtac gatttatggg
     1501 tataataata agctaaatcg agactaagtt ttattgttat atatattttt tttattttat
     1561 gcagaaatta attaaggttt ccgatggcac ctgtgtcaag gtcttccaac aactccgggt
     1621 cttccagcga cttcaagtct tccaataatc tcaaggtctt ccagataatc ctgagcttcc
     1681 agaaaatcca catcttccag acaatccatg tcttccggac aatccatgtc ttccaagaag
     1741 ctccaagtct tccagtaaat caagtcttcc agcaaatcca gtcttccagc aattactggt
     1801 cttccaccaa atccagatct tccaggaaaa tccacgtctt ccaggaaatc catgtcttcc
     1861 aataatttca aggtcttcca tcaaatacag atcttccagc taatccatgt cttccagaaa
     1921 aatctgtgtc ttccaccaaa tccaagtctt ccagtaaatc tagttcttcc agaaaaatct
     1981 agatcttcca gtcaatcagt gtcttccaga aagaaatcca ggtcttccag tcaatcagtg
     2041 tcttccagaa agaaatccag gtcttccagt cagtcagtgt cttccagaaa aatctacgtc
     2101 ttccaccaaa tccaggtctt ccagtcaatc cacatcttcc ggaaaaaatc caggtcttcc
     2161 agccaatata tgtcttcctg aagatccacg tcttccagaa aatccatgtc ttccagaaaa
     2221 tccatgtctt ccagtaacct cccagtcttc cagaaaatcc acgtcttccc aacaatccaa
     2281 gtcttccgga taatttgggt cttcctgaaa atctacgtct tccaaaaaag ccatgtcttc
     2341 cagaaaatcc acatcttcca atggcctcca ggtcttccag actatccatg tcttccagaa
     2401 aatccttgtc ttcccttaaa tctatagctt ccaaaaaatc cgggtcttcc aggaaatccg
     2461 tgtcttccag caagtccacg tcttccaaca aagccatgtc ttccagacta tccatgtctt
     2521 ccagaaaatc cttgtcttcc ctcaaatcca tagcttccga aaaatccagg tcttccagga
     2581 aatccgtgtc ttccagcaaa tccacgtctt ccaacaaagc catgtcttcc atcaaattaa
     2641 tgtcttccag cctacttgtg tcttccaaca aaggtacgtc ttccaacaaa ggtacgtctt
     2701 ccaacaaagg tatgtcttcc aacaaaggta cgtcttccag aaaatccacg tcttccaacc
     2761 aagccatgtc ttccagaaaa tccacgtctt ccagaaaata tatgtcttcc aactaagcta
     2821 cgtcttccaa caaatccatg tcttcctata tctccaggtc ttccagcatc tccagggctt
     2881 ccagcatctg ctcgtcttcc aacatctcca cgtcttccag catctctgtg tcttccagca
     2941 tcttcatgtc ttccaacaac tacccagtct tccatcaact ggctcaatat ccatgtcttc
     3001 caacgtctcc agtgtgctga tcttctgaca ttcaggtctt ccagtgtctg caatatccag
     3061 ccgcggagcg taagtattca aaattccaaa attttttact agaaatattc gattttttaa
     3121 taggcagttt ctatactatt gtatactatt gtagattcgt tgaaaagtat gtaacaggaa
     3181 gaataaagca tttccgacca tgtaaagtat atatattctt aataaggatc aatagccgag
     3241 tcgatctcgc catgtccgtc tgtcttatta ttttattacc gccgagacat caggaactat
     3301 aaaagctaga aggatgagtt ttagcataca gattctagag acaaggacgc agagcaagtt
     3361 tgttgatcca tgctgccacg ctttaacttt ctcaaattgc ccaaaactgc catgcccaca
     3421 tttttgaact attttcgaaa ttttttcata attgtattac tcgtgtaaat ttccatcaat
     3481 ttgccaaaaa actttttgtc acgcgttaac gccctaaagc cgccaatttg gtcacgccca
     3541 cactattgaa caattatcaa attttttctc attttattcc ccaatatcta tcgatatccc
     3601 cgattatgaa attattaaat ttcgcgttcg cattcacact agctgagtaa cgagtatctg
     3661 atagttgggg aaatcgactt attttttata tacaatgaaa atgaatttaa tcatatgaat
     3721 atcgattata gctttttatt taatatgaat atttatttgg gcttaaggtg taacctcctc
     3781 gacataagac tcacatggcg caggcacatt gaagacaaaa atactcattg tcgggtctcg
     3841 caccctccag cagcacctaa aattatgtct tcaattattg ccaacattgg agacacaatt
     3901 agtctgtggc acctcaggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
     3961 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4021 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4081 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4141 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     4201 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     4261 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4321 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4381 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4441 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4501 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4561 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4621 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     4681 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4741 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4801 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4861 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4921 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4981 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5041 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5101 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5161 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5221 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5281 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5341 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     5401 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     5461 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     5521 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5581 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     5641 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     5701 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5761 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5821 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     5881 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     5941 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6001 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6061 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6121 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6181 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6241 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6301 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6361 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7141 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7981 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8341 atttccccga aaagtgccac ctgacgtc
//
