LOCUS       sequence-127132-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging with mTFP1 using URA3 as a selection marker in S.
            cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: POM42-mTFP1
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..724)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
                     NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
                     DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
                     DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
                     ARNSTDGMDELYK"
     protein_bind    complement(763..796)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggccgcatag gccactagtc ttatacagct cgtccatgcc gtcggtggag ttgcgggcca
       61 cggcgctctc gtaaacggtc accttgttgt agtccttgtc gtggttcagg atctcgatgc
      121 ggtggtccac aaagtgatag tcgggcagct tcaccgcctt cttggccctg tagatggtct
      181 tgaagtcaac gcggtggtgg ccgccgccct ccagcagcag cttgtgcttg acgtcgccct
      241 tcagcacgcc gtcgcgcacg tacatcctct cggtggaggc gtcccagccg gtggtcttct
      301 tctgcatcac ggggccgttg ggggggaagt tctcgccctt gaggtgtatc tcgtagatga
      361 aggagtcctc ctccatggag atgtcggact tcaccttcac gatgcccttg tcctcgaagg
      421 tcatggtgcg ctcccaagag tagccctcgg ggaaggactg cttgaagtag ttggggatgt
      481 cgtcggggta cttggtgaag gccctgttgc cgtaggcgaa cgcggtggtc agaatgtcgt
      541 aggagaaggg caggggggct ccctccttca cctccaggtt gatggtgttg gtgccgtcgt
      601 agggcttgcc ctcgccctcg ccctcgatca cgaaggcgtg gccattcacg ttgccctcca
      661 tcttcagctt gatcttcatg tcgggcttga ttacgcccat tgtggtctcc tcgcccttgc
      721 tcacggatcc gtcgacctgc agcgtacgca tgatatcacc taataacttc gtatagcata
      781 cattatacga agttatatta agggttctcg agagctcgtt ttatttaggt tctatcgagg
      841 agaaaaagcg acaagaagag atagaccatg gataaatgat tatgttctaa acactcctca
      901 gaagctcatc gaactgtcat cctgcgtgaa gattaaaatc caacttagaa atttcgagct
      961 tacggagaca atcatatggg agaagcaatt ggaagataga aaa
//
