LOCUS       sequence-127133-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging with mTFP1 using URA3 as a selection marker in S.
            cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: POM42-mTFP1
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..70
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgaagcttcg tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat
       61 acgaagttat taggtctaga gatcccaata caacagatca cgtgatcttt ttgtaagatg
      121 aagttgaagt agtgttgcac cgtgccaatg caggtggcta ttagattaaa tatgtgattt
      181 gttctattaa gtttcctgta taattaatgg ggagcgctga ttctcttttg gtacgcttcc
      241 catccagcat ttctgtatct ttcaccttca accttaggat ctctaccctt ggcgaaaagt
      301 cctctgccaa caatgatgat atctgatcca ccacttacaa cttcgtcgac ggttctgtac
      361 tgctgaccca atgcatcgcc tttgtcgtct aaacctacac ctggggtcat gattagccaa
      421 tcaaaccctt cttctcttcc tcccatatcg ttctgagcaa tgaacccaat aacgaaatct
      481 ttatcactct ttgcaatatc aacggtaccc ttagtatatt caccgtgtgc tagagaaccc
      541 ttggaagaca attcagcaag catcaataat ccccttggtt ctttggtgac ctcttgcgca
      601 ccttgtttca agccagcaac aataccagca ccagtaaccc cgtgggcgtt ggtgatatca
      661 gaccattctg cgatacggta aacgcccgat gtatattgta atttgactgt gttaccgata
      721 tcggcgaatt ttctgtcctc aaatatcaag aacttgtatt tctctgccaa tgctttcaat
      781 ggaacgacag taccctcata actgaaatca tccaagatat caacgtgtgt tttcaaaagg
      841 caaatgtatg gacccaacgt ttcaacaagt ttcaatagct catcagtcga acgaacgtca
      901 agagaagcac acaaattggt cttcttttca tccattaaac gtaaaagttt cgatgcaacc
      961 ggacttgcat gagtctcagc tctactggta tatgattttg tggacatggt gcaactaatt
     1021 gacgggagtg tattgacgct ggcgtactgg ctttcacaaa atggcccaat cacaaccaca
     1081 tcttaga
//
