LOCUS       sequence-127153-        2199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pT181 STAR Antisense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL2154
FEATURES             Location/Qualifiers
     source          1..2199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..50
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(169..186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(340..928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(420..439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1099..1890,1891..1959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1722..1741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1960..2064)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2132..2150
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2154..2197)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattctaaa gatctttgac agctagctca gtcctaggta taatactagt aacaaaataa
       61 agcaataagg aatcgctcac ccaaatatat atctttatgt ggatctcaaa gcccgccgaa
      121 aggcgggctt ttttttggat ccttactcga gtctagactg caggcttcct cgctcactga
      181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc
      361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      781 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1021 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1081 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1141 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1201 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1261 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1321 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1381 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1441 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1501 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1561 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1621 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1681 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     1741 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     1801 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1861 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1921 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     1981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2101 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     2161 ataaaaaaaa tccttagctt tcgctaagga tgatttctg
//
