LOCUS       sequence-127156-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CDS of the Hygromycin resistance gene as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pHygromycin (GB0211)
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctccggccg ccatggcggc cgcgggaatt cgattgggcg atgagtggtc tcgaatgaaa
       61 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtg
      121 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
      181 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
      241 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
      301 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
      361 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
      421 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
      481 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
      541 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
      601 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
      661 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcaatgttc
      721 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
      781 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccacgactc
      841 cgggcgtata tgctccgcat tggtcttgac caactctatc aga
//
