LOCUS       sequence-127158-        2172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Anti-anti STAR Antisense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL1865
FEATURES             Location/Qualifiers
     source          1..2172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..50
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(142..159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(313..901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(393..412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1072..1863,1864..1932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1695..1714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1933..2037)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2105..2123
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2127..2170)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattctaaa gatctttgac agctagctca gtcctaggta taatactagt gactagacat
       61 aggaaaaagg agtcgatttc aaagcccgcc gaaaggcggg cttttttttg gatccttact
      121 cgagtctaga ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      301 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      961 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1021 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1081 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1141 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1201 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1261 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1321 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1381 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1441 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1501 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1561 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1621 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1681 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1741 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1801 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1861 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1921 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1981 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2041 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2101 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     2161 ggatgatttc tg
//
