LOCUS       sequence-127159-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging with mTFP1 using LEU2 as a selection marker in S.
            cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: POM43-mTFP1
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..721)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
                     NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
                     DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
                     DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
                     ARNSTDGMDELYK"
     protein_bind    complement(760..793)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcataggcc actagtcttg tacagctcgt ccatgccgtc ggtggagttg cgggccacgg
       61 cgctctcgta aacggtcacc ttgttgtagt ccttgtcgtg gttcaggatc tcgatgcggt
      121 ggtccacaaa gtgatagtcg ggcagcttca ccgccttctt ggccctgtag atggtcttga
      181 agtcaacgcg gtggtggccg ccgccctcca gcagcagctt gtgcttgacg tcgcccttca
      241 gcacgccgtc gcgcacgtac atcctctcgg tggaggcgtc ccagccggtg gtcttcttct
      301 gcatcacggg gccgttgggg gggaagttct cgcccttgag gtgtatctcg tagatgaagg
      361 agtcctcctc catggagatg tcggacttca ccttcacgat gcccttgtcc tcgaaggtca
      421 tggtgcgctc ccaagagtag ccctcgggga aggactgctt gaagtagttg gggatgtcgt
      481 cggggtactt ggtgaaggcc ctgttgccgt aggcgaacgc ggtggtcaga atgtcgtagg
      541 agaagggcag gggggctccc tccttcacct ccaggttgat ggtgttggtg ccgtcgtagg
      601 gcttgccctc gccctcgccc tcgatcacga aggcgtggcc attcacgttg ccctccatct
      661 tcagcttgat cttcatgtcg ggcttgatta cgcccattgt ggtctcctcg cccttgctca
      721 cggatccgtc gacctgcagc gtacgcatga tatcacctaa taacttcgta tagcatacat
      781 tatacgaagt tatattaagg gttctcgaga gctcgctgtg aagatcccag caaaggctta
      841 caaagtgtta tctcttttga gacttgttga gttgaacact ggtgttt
//
