LOCUS       sequence-127166-        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging with mTFP1 using LEU2 as a selection marker in S.
            cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: POM43-mTFP1
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..67
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcttcgtac gctgcaggtc gacaaccctt aatataactt cgtataatgt atgctatacg
       61 aagttattag gtctagagat ccgcaggcta accggaacct gtattattta gtttatgcta
      121 cgttaaataa agacctttcg ttcacataac tgaatgtgta atggccttga gatttcaagc
      181 ataccaagtt ggtggagacg gggtcgttac aaaagactct ttaagccaag atttccttga
      241 cagccttggc gatagcatcg ccaacctcag tggtagagtt agaaccacca aggtcaccgg
      301 ttctgacacc tgcatccaag acatttctaa cagcttcttc aagagcccta ccttcttcaa
      361 ccaaatccaa ggataacttc aacatcatag ctgcagataa gatggtagca attgggttaa
      421 ccttgtttgc tggtaaatct ggggcagaac catgacatgg ttcgtacaaa ccgaatgcct
      481 tgttagtgtc aggtagggaa gctagagatg cagaaggtaa taaacccaaa gaacctggaa
      541 taacagaggc ttcatcggag ataatatcac caaacatgtt gttggtaata acaacaccgt
      601 ttagcttagt tggtgattta accaaaatca tagcagcaga gtcgatcaat tggtgctgaa
      661 cagttaattg tgggaactca gtcttgatgg tttcttcaac agtctttctc cacaatctgg
      721 aagaggcaag cacgttagcc ttgtcaagag accagattgg taatggtggg ttttgttgca
      781 atgccaagaa agcagccatt cttgtaattc tttgaacttc aggaacactg tatttctcag
      841 agtcccaagc aactccgtca ccttcatctt cttttctttc accaaagtag ataccaccaa
      901 ccaattctct aacgacgacg aaatcggtac cctttgcata ttcaggcttc aaaggagaaa
      961 gatctagtaa agaatcagaa gcaaagttac atggtctcaa gttggcgtat agacccaatt
     1021 cctttctgat cttcaataga ccttgttctg gtctaactgc gcccgtaccc cattttggac
     1081 caccaacagc acctag
//
