LOCUS       sequence-127168-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging with mCitrine using URA3 as a selection marker in
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: POM42-mCitrine
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..733)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLEF
                     VTAAGITHGIDELYK"
     protein_bind    complement(772..805)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcggccgca taggccacta gttttgtaca attcatcaat accatgggta ataccagcag
       61 cagtaacaaa ttctaacaag accatgtggt ctctcttttc gtttggatct ttggataatc
      121 tagattgata ggataagtaa tggttgtctg gtaacaagac tggaccatca ccaattggag
      181 tattttgttg ataatggtca gctaattgaa cagaaccatc ttcaatgttg tgtctaattt
      241 tgaagttaac tttgatacca ttcttttgtt tgtcagccat gatgtaaaca ttgtgagagt
      301 tatagttgta ttccaatttg tgacctaaaa tgttaccatc ttctttaaaa tcaatacctt
      361 ttaattcgat tctattaact aaggtatcac cttcaaactt gacttcagct ctggtcttgt
      421 agttaccgtc atctttgaaa aaaatagttc tttcttgaac ataaccttct ggcatggcag
      481 acttgaaaaa gtcatgttgt ttcatatgat ctgggtatct agcaaaacac atcaaaccat
      541 aacctaaagt agtgactaag gttggccatg gaactggcaa tttaccagta gtacaaataa
      601 attttaaggt caatttaccg taagtagcat caccttcacc ttcaccggag acagaaaatt
      661 tgtgaccatt aacatcacca tctaattcaa ccaaaattgg gacaacacca gtgaataatt
      721 cttcaccttt agaggatccg tcgacctgca gcgtacgcat gatatcacct aataacttcg
      781 tatagcatac attatacgaa gttatattaa gggttctcga gagctcgttt tatttaggtt
      841 ctatcgagga gaaaaagcga caagaagaga tagaccatgg ataaatgatt atgttctaaa
      901 cactcctcag aagctcatcg aactgtcatc ctgcgtgaag attaa
//
