LOCUS       sequence-12718-e        7209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pBF3038
FEATURES             Location/Qualifiers
     source          1..7209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2..119
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     414..437
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      1627..1874
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1643..1661)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1944..1961)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2115..2703)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2195..2214)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2874..3665,3666..3734))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(3752..4846)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      5788..6668
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(6737..7192)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     7086..7105
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gccatggaaa
      481 aaaatgtcta atttgttgac tgttcatcaa aatttgccag ctttgccagt tgatgcaacg
      541 tctgatgagg taagaaagaa tttgatggat atgtttagag atagacaagc attctctgaa
      601 catacttgga aaatgttgtt gtctgtttgt agatcttggg ctgcttggtg taaattaaat
      661 aatagaaaat ggtttccagc tgaaccagaa gatgttagag attatttgtt gtatttgcaa
      721 gctagaggtt tggctgttaa aactatacaa caacatttgg gtcaattaaa tatgttgcat
      781 aggagatctg gtttgcctag accatctgat tctaatgctg tatctttggt tatgagaagg
      841 attagaaaag aaaatgttga tgcaggtgaa agagctaaac aagcattggc gtttgaaaga
      901 actgattttg atcaagttag atctttgatg gaaaattctg atagatgtca agatataaga
      961 aacttggctt tcttgggaat tgcttataat acattgttga gaattgctga aattgctaga
     1021 attagagtta aagacatttc tagaactgat ggtggtagaa tgttgattca tattggtaga
     1081 actaaaacat tggtttcaac tgctggcgtt gagaaagcat tgtctttggg tgttactaaa
     1141 ttggttgaaa gatggatttc tgtatctggt gtggctgatg acccaaataa ttatttattt
     1201 tgtagagtta gaaagaatgg tgttgcggct ccatctgcta catctcagct ctctacccgt
     1261 gccttggaag gaatttttga agcaactcat agattgattt atggtgctaa agatgattct
     1321 ggtcaaagat atttagcatg gtctggtcat tctgctagag ttggtgccgc tagagatatg
     1381 gctagagctg gggtatctat tcctgaaatt atgcaagctg gtggttggac taatgttaac
     1441 atcgtcatga actatattag aaatttggat tctgaaactg gtgcaatggt tagactcttg
     1501 gaagatggtg atcccgggaa gcttggtacc gagctcggat ccactagtaa cggccgccag
     1561 tgtgctggaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg
     1621 gccgcatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc
     1681 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
     1741 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac
     1801 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     1861 aggctttaat ttgcggccct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1921 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1981 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2041 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagc ccaggaaccg taaaaaggcc
     2101 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2161 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2341 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2401 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2461 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2521 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2581 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2641 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2701 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2761 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2821 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2881 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2941 tgactccccg tcgtgtagat aactacgata cgggagcgct taccatctgg ccccagtgct
     3001 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3061 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat tcagtctatt
     3121 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3181 ggcattgcta caggcatcgt ggtgtcactc tcgtcgtttg gtatggcttc attcagctcc
     3241 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3301 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3361 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3421 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3481 ccggcgtcaa tacgggataa tagtgtatca catagcagaa ctttaaaagt gctcatcatt
     3541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3721 tgttgaatac tcatactctt cctttttcaa tttaagcaag gattttctta acttcttcgg
     3781 cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac
     3841 ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca
     3901 atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg accttattct
     3961 ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt
     4021 ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg
     4081 cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg
     4141 ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg
     4201 taggaaattc gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca
     4261 aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga
     4321 aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag
     4381 cgacaccatc accatcatct tcctttctct taccaaagta aatacctccc actaattctc
     4441 tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa
     4501 gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga
     4561 tttttagtaa accttgttca ggtctaacac tacctgtacc ccatttagga ccacccacag
     4621 cacctaacaa aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggga
     4681 cacctgtagc ttcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat
     4741 tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac
     4801 caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc
     4861 cttgaaatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag aaagtaagac
     4921 gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt caacttcaag
     4981 tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc
     5041 ggcctctcac ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgtcaggc
     5101 gacctctgaa attaacaaaa aatttccagt catcgaattt gattctgtgc gatagcgccc
     5161 ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggtta tcgatgataa gctgtcaaag
     5221 atgagaatta attccacgga ctatagacta tactagatac tccgtctact gtacgataca
     5281 cttccgctca ggtccttgtc ctttaacgag gccttaccac tcttttgtta ctctattgat
     5341 ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt aaaactagct
     5401 agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc atcattatta
     5461 tccgatgtga cgctgcagct tctcaatgat attcgaatac gctttgagga gatacagcct
     5521 aatatccgac aaactgtttt acagatttac gatcgtactt gttacccatc attgaatttt
     5581 gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa
     5641 tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt
     5701 gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat
     5761 cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa
     5821 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     5881 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
     5941 aacaaaaatg caacgcgacg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     6001 ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt
     6061 ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta
     6121 ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg
     6181 tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct
     6241 gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata
     6301 aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag
     6361 tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc
     6421 tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga
     6481 atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg
     6541 tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg
     6601 gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta
     6661 ttcgcaatgg gaagctccac cccggttgat aatcagaaaa gccccaaaaa caggaagatt
     6721 gtataagcaa atatttaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     6781 tgttaaatca gctcattttt taacgaatag cccgaaatcg gcaaaatccc ttataaatca
     6841 aaagaataga ccgagatagg gttgagtgtt gttccagttt ccaacaagag tccactatta
     6901 aagaacgtgg actccaacgt caaagggcga aaaagggtct atcagggcga tggcccacta
     6961 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc agtaaatcgg
     7021 aagggtaaac ggatgccccc atttagagct tgacggggaa agccggcgaa cgtggcgaga
     7081 aaggaaggga agaaagcgaa aggagcgggg gctagggcgg tgggaagtgt aggggtcacg
     7141 ctgggcgtaa ccaccacacc cgccgcgctt aatggggcgc tacagggcgc gtggggatga
     7201 tccactagt
//
