LOCUS       sequence-127189-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging with mCherry using LEU2 as a selection marker in 
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: POM43-mCherry
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..727)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(298..317)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     567..585
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(766..799)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcggccgca taggccacta gtcttgtaca gctcgtccat gccgccggtg gagtggcggc
       61 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      121 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
      181 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
      241 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct
      301 tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga
      361 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
      421 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
      481 tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt
      541 cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct
      601 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
      661 ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct
      721 tgctcacgga tccgtcgacc tgcagcgtac gcatgatatc acctaataac ttcgtatagc
      781 atacattata cgaagttata ttaagggttc tcgagagctc gctgtgaaga tcccagcaaa
      841 ggcttacaaa gtgttatctc ttttgagact tgttgagttg aacactggtg ttttcatcaa
      901 acttaccaag gacgtgtacc cattgttgaa acttgtatca ccatatattg ttatcggaca
      961 accttcactt gcatctatcc gttctttaat ccaaaa
//
