LOCUS       sequence-12719-e       12577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12577)
  TITLE     Christopher Potter Lab Plasmids
REFERENCE   2  (bases 1 to 12577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGal80,y+
FEATURES             Location/Qualifiers
     source          1..12577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             839..2146
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL80"
                     /product="transcriptional repressor that inhibits GAL4"
                     /label=GAL80
                     /note="GAL80 binds to and inhibits the transcriptional 
                     activation domain of GAL4."
                     /translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSS
                     QFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEVVM
                     PLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKE
                     LISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMT
                     SSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKG
                     GKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLD
                     PGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGF
                     PTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
     primer_bind     complement(2408..2424)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2408..2424)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             8870..8881
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10331..10349)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10417..10521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10522..10590,10591..11382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMSVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10740..10759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11553..12141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12042..12061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(12343..12544,12545..12575)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 cgttcccatg tgttggtctg tatgccgtag tgctgccgtc gcagcggttt tcacgccaaa
       61 tctggcttgt ttcagaggtc aggaactaac ttagtctgca ggtgtagtta gcatgttccc
      121 gcctacacag gttcactgaa caaaagtatt taaacataaa aatatctatt ttatagatac
      181 aatttttctt gttcttatag tttttactaa gaaaacgatt agttataaaa aatattatgt
      241 aagtgatgtc aaaaaagaaa gtagcatgtc gttggtattc ttttcataat tgagttcaag
      301 gctaaacttt ttctattcca tatattttta aataatttat atcattaccc gaattatatt
      361 tcaaaggaaa aacagttagg taaactttct tctattatat ttttactctt taaaaacttt
      421 cctatgcatt gtagtaacgt tttagatttt tttttgccta tttatttaaa cataaatcag
      481 caaattctat tttattcata aatgtcccac caaccaatgt tcttcaagac aatagcctac
      541 agcactagtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg
      601 atctgccgct ggactacgtg ggtctggccc atgatgaaat aacataaggt ggtcccgtcg
      661 atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt gttgagagga
      721 aaggttgtgt gcggacgaat ttttttttga aaacattaac ccttacgtgc ggccgctaag
      781 caaataaaca agcgcagctg aacaagctaa acaatctgca gcccaagctg gccgcaacat
      841 ggactacaac aagagatctt cggtctcaac cgtgcctaat gcagctccca taagagtcgg
      901 attcgtcggt ctcaacgcag ccaaaggatg ggcaatcaag acacattacc ccgccatact
      961 gcaactatcg tcacaatttc aaatcactgc cttatacagt ccaaaaattg agacttctat
     1021 tgccaccatt cagcgtctaa aattgagtaa tgccactgct tttcccactt tagagtcatt
     1081 tgcatcatct tccactatag atatgatagt gatagctatc caagtggcca gccattatga
     1141 agttgttatg cctctcttgg aattctccaa aaataatccg aacctcaagt atcttttcgt
     1201 agaatgggcc cttgcatgtt cactagatca agccgaatcc atttataagg ctgctgctga
     1261 acgtggggtt caaaccatca tctctttaca aggtcgtaaa tcaccatata ttttgagagc
     1321 aaaagaatta atatctcaag gctatatcgg cgacattaat tcgatcgaga ttgctggaaa
     1381 tggcggttgg tacggctacg aaaggcctgt taaatcacca aaatacatct atgaaatcgg
     1441 gaacggtgta gatctggtaa ccacaacatt tggtcacaca atcgatattt tacaatacat
     1501 gacaagttcg tacttttcca ggataaatgc aatggttttc aataatattc cagagcaaga
     1561 gctgatagat gagcgtggta accgattggg ccagcgagtc ccaaagacag taccggatca
     1621 tcttttattc caaggcacat tgttaaatgg caatgttcca gtgtcatgca gtttcaaagg
     1681 tggcaaacct accaaaaaat ttaccaaaaa tttggtcatt gacattcacg gtaccaaggg
     1741 agatttgaaa cttgaaggcg atgccggctt cgcagaaatt tcaaatctgg tcctttacta
     1801 cagtggaact agagcaaacg acttcccgct agccaatgga caacaagctc ctttagaccc
     1861 ggggtatgat gcaggtaaag aaatcatgga agtatatcat ttacgaaatt ataatgccat
     1921 tgtgggtaat attcatcgac tgtatcaatc tatctctgac ttccacttca atacaaagaa
     1981 aattcctgaa ttaccctcac aatttgtaat gcaaggtttc gatttcgaag gctttcccac
     2041 cttgatggat gctctgatat tacacaggtt aatcgagagc gtttataaaa gtaacatgat
     2101 gggctccaca ttaaacgtta gcaatatctc gcattatagt ttataatcta gagcttatcg
     2161 ataccgtcga ctaaagccaa atagaaaatt attcagttcc tggcttaagt ttttaaaagt
     2221 gatattattt atttggttgt aaccaaccaa aagaatgtaa ataactaata cataattatg
     2281 ttagttttaa gttagcaaca aattgatttt agctatatta gctacttggt taataaatag
     2341 aatatattta tttaaagata attgcgtttt tattgtcagg gagtgagttt gcttaaaaac
     2401 tcgtttagat ccactagttc tagagcggcc gcagcttgat tcgactatta aatgattatc
     2461 gcccgattac cacattgagt ggtttaaaat agccataaaa tatgcaactg acgatggctt
     2521 aagataaata cgtcgcagag tcactcataa atttcgaacg cagcccgctg atttacctac
     2581 ccctctaaac gattcatagt atatgtacga gtatatccac taagcttttt cgagcactga
     2641 ttttttcgct tgcacgagac aagtgcacca ccgcaattgc aggcaaatta tgtctgaggt
     2701 aatgattccg tttcgtgcaa gattacacag aaatcaaatt acgacaacct ttattcagta
     2761 agcaaacaaa gcctttgttg gcatctaatt attccactta tggttgcgat ttcgggagct
     2821 acaatcggtt ttggtttagt atatctagcg agttccttgg cgacatttaa aatttacaaa
     2881 taaagtttct ctattcaatc gggacagtgg aaattgacta ttttatttat attaatgaac
     2941 ttatttttaa tttggcttaa gttactaagg ggtactaata gtttgagcgc agtgcatgtc
     3001 atggggacat gtgcaattgt gtgtaagcgg gaagtgatcg cggccttccg aatttggcca
     3061 tgccaaataa tcccagctcg aaaggagggg acccggcggt cagggccatg gacattgaac
     3121 ttgaaaaaaa aaaaaacaca aaaatatata acacaaaacg gaaaatgctg tgtaccgctt
     3181 atgttagaga agttgagcaa cgggtttttc gttttgcagt cacgatggat ttccaaatta
     3241 gtgtaggagg ggggagggga gggagggaga taatgtccag gctgccataa gtggggaata
     3301 aggaaaataa aacatgaaac acgggtcggg caatgtcatg cggtattcgg ctttgctttc
     3361 cgcccaagtt gaagtgatcc tgtgtgtaaa taatgtcgaa tgttgccggt cggttgcata
     3421 agcgttagtc aattatgcca aagagatctg atttgtggaa gctttttttg accacttagc
     3481 gcgctccgct tgatgttgtt ttgttttgtg ctggggcaga aaacttgttt caattattgg
     3541 gaaaagtgcg tataaatcat tgccgcaagc tctgaaaagc gaaaaagaaa aacagtaacc
     3601 aaacagacaa acgcagcatc cccccacaca attaagcaaa aacttgaaaa caagtcaatt
     3661 cgaaaaaaat tataggttca acggctgcag cgatcgcatc attagttgcg tttttagtaa
     3721 atacaccatt tcattacaca acacacacaa ttaattaata aaactgtact ggttatttca
     3781 agtgtgtctt ttaataagcc tgccgatcgc aataaattcg agcagcattg ccggtaattt
     3841 tgtgcaacat atttttcgat tgccacaccg tgtttgttta tttttctgtg ggtgcaatga
     3901 tttagaatgc gggcaaggga tcaagttgaa ccacttctaa gaaaaaatag cattgcataa
     3961 atgatataga gtccaaaaac tacacaaatt caatagcagt aatggttaca ttagctttga
     4021 aattgttttt agacatccga agaaataaga ttaaatttaa acggcattct ttaatttgta
     4081 ttttaatatt ttgagaggtt ttccttattt aaagtgtaga ttattgagga ttaatgcaaa
     4141 ccactttatc tgcggaggtc gtaaaacgta tttttaccca tttgcatgtt tattatgcgt
     4201 gtggctggtt gtattacttt acttaagttt tgcaattttt tctttagcaa gcaggtgcat
     4261 ttgggccaag agatatatgc gatcgctttc ggttcgaatt tttaacattt acttgcggcg
     4321 atggtcatta gagcattacc cacttagggc acccccaaca tccagttgat tttcagggac
     4381 cacaatattt taaataacag ctagtggaat tacctaaaag cgctttcgtc ccttttgaaa
     4441 ttttatgtaa cactcaatta tatttatgta tatgtatgct caaaatcacc tgccaataac
     4501 tagcggaaac caaatatttg accctcagtg aattgtgaat catcggtgac gcccaatcga
     4561 aatccaatcc taagcaattg aaacgagcac gagttccaat ttaatagtat acaaggaaac
     4621 acctgcttta aatactctac atagtacacg ttataataac gatttatttg atatttctgg
     4681 atttttgtct gcatgtattt catataatat tgatttgatt tttttaatga attgaactaa
     4741 aaaatcatat tagaacattt tttgcagtcg ccgataaaga tgaacactgt tctcagaaca
     4801 caactgtcat gtattaagct ttcagatttt cagaaatttg gagagcaatg cattctatgc
     4861 acgagcctcc tggccttaca atttacttgt ttgaaattag atcgtcaaat aaagtcccta
     4921 aaattaaata aatagtagtc acaactttaa aataggtctt aatcttttag ggtaccgaaa
     4981 ggtatttcgg cacaaatcag cgcagtttta aatgtcgatg aaggccaaaa atcataccaa
     5041 acccagcgaa aggtgatgtc tgactcatta aattggggga ttcgagtgta tttattaaac
     5101 atgcgtgaaa atcaatcatg gaagacaaaa cgcaaagttg gccgatctat gggaacagca
     5161 taagccacct gattacccga acactgaacc acccgaatca ctaaaaccac cgaagttggc
     5221 gcgcgccttc gttttcattt tcattggcct gtcttcgtct tcggagaaaa aaaacttcat
     5281 ataaaacgcg gccgacatat tatggccacc agtcgttacc gcgccacggt ccacagaaga
     5341 ggattaaaaa aatatcacac agccgaaggc tagagaagaa ccccctatag ctgaacatat
     5401 ataaacaaat atattttttt ttattgccaa cacactttgg cttaagtgtt aagagtgatt
     5461 gtcagcttag agctaagtgc actgttccag gacaaagggt ggatccttgt gaccctgatc
     5521 accttggtga cgccgtcttg ggctgcttac aaacttcagg agcgatatag ttggagccag
     5581 ctggactttg ctttcccgaa tacccgacta aaggaccaag ctctggctag tggagattat
     5641 attccgcaaa atgctctacc tgttggagtc gaacactttg gcaatcggtt attcgtcact
     5701 gttccccgct ggcgtgatgg taagtggaag ttaaatatga agcccttggg gagatcgtaa
     5761 atgggacatt cttacttagg gcatcagaga tatctgattg agtggttgac agttttatat
     5821 ggcttgtttg acatgatgta aaaacacaaa attcatttag tttaggtatt caaataagag
     5881 cttgttattt attttagaat ttggagaaca tttttttgtc tttctaccct tcttagaaaa
     5941 taatattgtt ttgtacaatt taattttaaa ctagtacaga cgaaaaatgt atttttttat
     6001 ttgtatgcct tttcaccatt ttggcagagg aataaatatg acaatatatt ttgagagcac
     6061 cctcatgtaa aggttttagc gtggcgacct ctcataaatc cggttggtac ctgcgcgtta
     6121 ttttaacatt ttaaacaatt aaccgttgta aaatcgaagc caatagcatg gcattggctt
     6181 tatactgtat taaattgtat tatattacca tccgaattgt aaagacttct tcagggccgc
     6241 cacatagaaa tggaaatcca atcacaaaca ataacttatg gcattagcta ttaaccgacg
     6301 attagctgtc agttcaacaa atgtaagagt ggcgaaatgt ttaaatgcga aggcattgtt
     6361 ctgtgactca cgttttatta ttaatcacac aaatgatttt gcttcaaaat tatttggctt
     6421 acacaataat caaaattttt atgaaatagt tgaaacacaa aactaggaaa ttttaaaaag
     6481 caatgaaact aaaaaaccca attgctaaga ttatatgatg cgcatacaaa tacttcagta
     6541 cgtctaggaa tgctttcgat gattgattag tttttatgca tggcttacaa ttggtattta
     6601 cacagaaaaa cacggcgatg ttaataaatt ttgtacatat gttcttaagc agtccgaaac
     6661 acccaaactt ctgactaaac ttaaaaaaac acgctcttca aggatatctt aatgtcactt
     6721 ataatgtaat ggtattgtat tattaagtat atcaaaatat tctggcccag ccttgaggtc
     6781 tctttttaaa aagatatcga ctgactacct ccagtcaatg aaataatagc ccagaaggcc
     6841 gaatcggcaa aaaataaacc ccaagttacg gcaaacaaaa catagtgaaa gttgtggcaa
     6901 agtggaacat ttaaaggcat gcttcaatgg ccatcgaagc aaatcaatta gtcaaagcaa
     6961 atcggtagtg gcaacaacag gctacagaat acctataagt gacagttatg gggtatgatt
     7021 aattataaat attatcattg accaccaatg ctgggctcaa ttggaaaaac tattctatga
     7081 agatttgagt aaataaattt tgatttaaaa aagcccatgg ttatcgcgac aactagctac
     7141 gggacaagat tactgtttaa aatcaagtgt gaaatatcaa aatcaaaatc ggattccgat
     7201 cgggaagttg tatccgattc tgaaactaaa acacagaatt gccaacattt tccgatatcg
     7261 actcagctca cgtatttcat acagattcat taggccacca gccattgaat aatatacccc
     7321 agtcaattga gctactcgat agttgatcaa cttagctttt gtcaacgagt gaacgcataa
     7381 actactacat caacgatatt tgcggcccat tccaagctaa aagttcatct taattacaaa
     7441 taagattaga aaaaatatct gaatgaaaaa aatgttgaga catatttctt tggaaaagga
     7501 gaacctcaag acagtcgaaa aaattgttta caatgaaaat gttgaaaatc atgaagcaga
     7561 taaatctgtc agttgcgagg ttttaggact gaaagagcac atgtcaaaat ataaatttgt
     7621 tcaaatactt tatatttgac tgaattagat tgttatttta aaagttatga attaaataaa
     7681 gattgaaagg tgcattatgc tcaaatgtat atttatcgca acccccggtt actttgtaaa
     7741 gcaaaaacgc ctggtttgat ttttaagaag atgggtcggt aaatcgataa aagctatatt
     7801 ttctggtcgt tgcagtctca ctcgcctgct ataaaaacat taaaagttcc cagaaacaat
     7861 aaatgtcttt aaattcaatt aacgaagaaa taaagaagga aaagaactgg agcggaaatc
     7921 ggtcgaaata ctgccaatgg ccacatatac atttaacagc gatatatggt atacatattg
     7981 ataatgatgt cagacgcaat tgcttcagac ggctaatgac atcgcaaatt gcacgcaact
     8041 tgcaatagtg ccaattatga ctgaagtaca tatagccggg gatcttttaa cataaacttc
     8101 cagtagatgt acaagcagaa aaaagagcca ttagcacggc agttaccatt gcttatgatt
     8161 ccttgtgtcc aaaataatga caaataggta tataaataat taaatgccaa acataagcga
     8221 ttctaattta cctttacatc tgtatgcatt tacatattat ccagaaaaca gacagcgata
     8281 acttgcaaca ttgcttagta taataatcca aagaaggaat ttaggcagaa attccagtta
     8341 attaaatatt caaaacaaac tttatttagt gcctcaataa tagtttggcc ctgctaattc
     8401 tcctatttta ttttttaggg attccggcca ctctgaccta tataaacatg gaccgcagtt
     8461 tgacgggttc accggagcta attccgtatc cagattggcg ctcaaataca gctggagatt
     8521 gcgccaacag tattaccact gcctaccgca ttaaagtgga tgagtgtggt cggctgtggg
     8581 ttttggacac tggaaccgtg ggcatcggca ataccaccac taatccgtgc ccctatgcgg
     8641 taaatgtctt tgacttgacc acggatacgc gaattcggag atacgagcta cctggcgtgg
     8701 acacaaatcc aaatactttc atagctaaca ttgccgtgga tataggcaaa aattgcgatg
     8761 atgcatatgc ctattttgcc gatgaattgg gatacggctt gattgcttac tcctgggaac
     8821 tgaacaagtc ctggagattc tcggcacatt cgtatttttt ccccgatcca ttgaggggcg
     8881 atttcaatgt cgctggtatt aacttccaat ggggcgagga gggtatattt ggtatgtccc
     8941 tttcgcccat tcgatcggat ggttatcgta ccctgtactt tagtccgtta gcaagtcatc
     9001 gacaatttgc cgtatccacg aggattttga gggatgaaac caggacggaa gatagctatc
     9061 atgactttgt tgccttagat gaacggggtc caaactccca taccacttca cgtgtgatga
     9121 gcgatgatgg aattgagctg ttcaatttaa tagatcaaaa tgcagtgggt tgctggcact
     9181 catcaatgcc gtactcaccg caatttcatg gcattgtgga tcgcgatgac gttggcttag
     9241 tttttccggc cgatgtgaaa attgatgaga acaaaaacgt ttgggttcta tccgatagga
     9301 tgcccgtttt cttgctgtct gacttggatt attcagatac taatttccga atttacacgg
     9361 ctcccttggc cactttaatt gagaatactg tgtgtgattt gaggaataac gcctatgggc
     9421 cgccaaatac cgtttcaata ccaaaacaag ccgttttgcc aatgggtcca ccgttatata
     9481 cgaaacaata tcgtcctgtc ttgccacaga aacctcagac cagctgggct tcctcgccgc
     9541 ctcctccaag tcgcacttat ttgcccgcca attcaggcaa tgtagtctcc agtattagtg
     9601 tctctacaaa ttctgtgggt cctgcaggag tggaggtgcc aaaggcctat attttcaacc
     9661 agcacaacgg cataaattac gagacaagtg gtccccatct atttcccacc catcaacccg
     9721 cccaaccggg tggccaggat ggtgggttaa aaacttatgt gaatgcccgc caatctgggt
     9781 ggtggcatca tcagcatcaa ggttaacata atcctacaca cggtacttgg gtatattctc
     9841 acacactcga ttgatgtaaa gaatatttaa agacaacaac atagggcaac agcggttaaa
     9901 aaaaccacat gacgtatgag caagtggcaa atcaatactt tatctagtta tgttaagcaa
     9961 aaaataacaa taaatcaact tttttttgaa ggttaagagt ttacgcaatt ttcttgagcg
    10021 gaaaaagcgg aaaaaatgta agtatgcata aattctaaat atatcaacaa ctgtacattt
    10081 tctggagtac tactaccagg caagaaagta ggttgataaa gctatgcaca agatcttgtt
    10141 tgggtgcagg gaaagttcaa cttaatcgct caatttgaga tcgcctggtc ccttgagatt
    10201 cgactgtaat tgaaattttt gcttttgatc ggagccagac ttcagacggg gcaaacaaaa
    10261 agactttgtt ggtggtaggg taggatccgt tgacctgcag gtcgaggaat taattcttga
    10321 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
    10381 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    10501 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    10561 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    10621 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10681 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10741 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
    10801 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10861 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10921 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10981 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    11041 gacgagcgtg acaccacgat gtctgtagca atggcaacaa cgttgcgcaa actattaact
    11101 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    11161 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    11221 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    11281 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    11341 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    11401 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11461 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11521 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11581 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11641 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    11701 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11761 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11821 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11881 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11941 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    12001 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    12061 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    12121 ggggggcgga gcctatggaa aaacgccagc aacgcaagct tctagagatc tgcaggtcga
    12181 cggatccgtc gagcgtataa ccatctgtac aaaaaaggat ttcctttgcc cagtcgtacg
    12241 actttgtaca gatggttatc agatgtggac ataaaaagag gatgtttgga tgtggtcata
    12301 gacctaatgg acagtgatgg agttgatgac gccgacaagc tttgcgtact cgcaaattat
    12361 taaaaataaa actttaaaaa taatttcgtc taattaatat tatgagttaa ttcaaacccc
    12421 acggacatgc taagggttaa tcaacaatca tatcgctgtc tcactcagac tcaatacgac
    12481 actcagaata ctattccttt cactcgcact tattgcaagc atacgttaag tggatgtctc
    12541 ttgccgacgg gaccacctta tgttatttca tcatggg
//
