LOCUS       sequence-127190-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging with mCherry using LEU2 as a selection marker in 
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: POM43-mCherry
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..70
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgaagcttcg tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat
       61 acgaagttat taggtctaga gatccgcagg ctaaccggaa cctgtattat ttagtttatg
      121 ctacgttaaa taaagacctt tcgttcacat aactgaatgt gtaatggcct tgagatttca
      181 agcataccaa gttggtggag acggggtcgt tacaaaagac tctttaagcc aagatttcct
      241 tgacagcctt ggcgatagca tcgccaacct cagtggtaga gttagaacca ccaaggtcac
      301 cggttctgac acctgcatcc aagacatttc taacagcttc ttcaagagcc ctaccttctt
      361 caaccaaatc caaggataac ttcaacatca tagctgcaga taagatggta gcaattgggt
      421 taaccttgtt tgctggtaaa tctggggcag aaccatgaca tggttcgtac aaaccgaatg
      481 ccttgttagt gtcaggtagg gaagctagag atgcagaagg taataaaccc aaagaacctg
      541 gaataacaga ggcttcatcg gagataatat caccaaacat gttgttggta ataacaacac
      601 cgtttagctt agttggtgat ttaaccaaaa tcatagcagc agagtcgatc aattggtgct
      661 gaacagttaa ttgtgggaac tcagtcttga tggtttcttc aacagtcttt ctccacaatc
      721 tggaagaggc aagcacgtta gccttgtcaa gagaccagat tggtaatggt gggttttgtt
      781 gcaatgccaa gaaagcagcc attcttgtaa ttctttgaac ttcaggaaca ctgtatttct
      841 cagagtccca agcaactccg tcaccttcat cttcttttct ttcaccaaag tagataccac
      901 caaccaattc tctaacgacg acgaaatcgg taccctttgc atattcaggc ttcaaaggag
      961 aaagatctag taaagaatca gaagc
//
