LOCUS       sequence-127196-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GST tagged GIV-CT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Garcia-Marcos M, Ghosh P, Ear J, Farquhar MG
  TITLE     A structural determinant that renders G alpha(i) sensitive to 
            activation by GIV/girdin is required to promote cell migration.
  JOURNAL   J Biol Chem. 2010 Apr 23;285(17):12765-77. doi: 
            10.1074/jbc.M109.045161. Epub 2010 Feb 15.
  PUBMED    20157114
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.045161"; journalName: "J Biol Chem"; date: 
            "2010-04-23- 23"; volume: "285"; issue: "17"; pages: "12765-77"
COMMENT     Sequence Label: GST-GIV-CT
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(47..52,53..68,69..75)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    83..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     88..110
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tgcgccgaca tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca
       61 tcggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagtatt
      121 catgtcccct atactaggtt attggaaaat taagggcctt gtgcaaccca ctcgacttct
      181 tttggaatat cttgaagaaa aatatgaaga gcatttgtat gagcgcgatg aaggtgataa
      241 atggcgaaac aaaaagtttg aattgggttt ggagtttccc aatcttcctt attatattga
      301 tggtgatgtt aaattaacac agtctatggc catcatacgt tatatagctg acaagcacaa
      361 catgttgggt ggttgtccaa aagagcgtgc agagatttca atgcttgaag gagcggtttt
      421 ggatattaga tacggtgttt cgagaattgc atatagtaaa gactttgaaa ctctcaaagt
      481 tgattttctt agcaagctac ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa
      541 aacatattta aatggtgatc atgtaaccca tcctgacttc atgttgtatg acgctcttga
      601 tgttgtttta tacatggacc caatgtgcct ggatgcgttc ccaaaattag tttgttttaa
      661 aaaacgtatt gaagctat
//
