LOCUS       sequence-127197-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express His tagged GIV-CTs (1660–1870)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Garcia-Marcos M, Ear J, Farquhar MG, Ghosh P
  TITLE     A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G 
            protein activity and growth factor signals.
  JOURNAL   Mol Biol Cell. 2011 Mar 1;22(5):673-86. doi: 
            10.1091/mbc.E10-08-0738. Epub 2011 Jan 5.
  PUBMED    21209316
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E10-08-0738"; journalName: "Mol Biol Cell"; date: 
            "2011-03-1- 1"; volume: "22"; issue: "5"; pages: "673-86"
COMMENT     Sequence Label: His-GIV-CTs
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     285..304
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     388..407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        388..406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    407..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             446..468
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             487..504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             514..531
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      181 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      241 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      301 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      361 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      421 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc
      481 agcagccatc atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatgtct
      541 gaaacactgg agagtcgaca tcacaagatc aaaactggtt cccctggaag tgaagttgtt
      601 actctacaac agtttttgga agaaagcaat aagcttacct cagtacagat aaagtcctca
      661 agtcaagaga atcttttaga tgaagtaatg aaaagtttgt ctgtctcttc tgactttttg
      721 ggaaaagaca aaccagttag ctgtggtctg gccaggtcag taagtggaaa aaccccaggg
      781 gacttctatg atagacggac aactaagcct gagtttt
//
