LOCUS       sequence-127198-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express His tagged GIV-CTs (1660–1870)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Garcia-Marcos M, Ear J, Farquhar MG, Ghosh P
  TITLE     A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G 
            protein activity and growth factor signals.
  JOURNAL   Mol Biol Cell. 2011 Mar 1;22(5):673-86. doi: 
            10.1091/mbc.E10-08-0738. Epub 2011 Jan 5.
  PUBMED    21209316
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E10-08-0738"; journalName: "Mol Biol Cell"; date: 
            "2011-03-1- 1"; volume: "22"; issue: "5"; pages: "673-86"
COMMENT     Sequence Label: His-GIV-CTs
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(56..103)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     99..117
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(170..187)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgccgctac agggcgcgtc ccattcgcca atccggatat agttcctcct ttcagcaaaa
       61 aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc tcagcggtgg
      121 cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatctcag tggtggtggt
      181 ggtggtgctc gagtgcggcc gcaagcttgt cgacggagct cgaattctta ttaggagctt
      241 tgttgctccc tagacctgct ttttgaattt ctgctttctt gtactttgtc cacattagat
      301 gctgcagtgg tgttgctgtc agcagcagct ggtggtgaat caactgatat aggcagtcta
      361 ctgtccttgg tcaaaaaatc atggatgctt gtccttcgtg tagttccttc ggcagttgag
      421 atcacgctgc ttgcacgagg taaagttgca taagggttac tatcttttga ttgtcgtgac
      481 agagaagatt cttttactaa ttttattttt ccttgagtgc ctggtgtagg ttttcccgca
      541 gaactaatga agtaggtatc ttcagttttt cgaggaccag gtctcaaaaa ctcaggctta
      601 gttgtccgtc tatcatagaa gtcccctggg gtttttccac ttactgacct ggccagacca
      661 cagctaactg gtttgtcttt tccca
//
