LOCUS       sequence-127206-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG303GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..231)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             272..361
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(406..424)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(445..461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(445..461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(458..480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    469..485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(493..499,500..517,518..523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    538..559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(676..693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
       61 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
      121 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      181 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tgatgggctg
      241 caggaattcg atatcaagct taggtggaat gtacccatac gatgttcctg actatgcggg
      301 ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc
      361 tgctcagtgc taactcgagg gggggcccgg taccagcttt tgttcccttt agtgagggtt
      421 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagg
//
