LOCUS       sequence-127222-         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-Cerulean-ccdB
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..133
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg
       61 aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa
      121 gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa
      181 aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa
      241 acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac
      301 ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt
      361 atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc
      421 gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga
      481 aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa
      541 agtatgcgca atccacatcg gtatagaata taatc
//
