LOCUS       sequence-127224-         577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-Cerulean-ccdB
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..134
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg cagctcgcgt tgcattttcg
       61 gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc gaagttccta ttctctagaa
      121 agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa
      181 aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac cgcttccaca
      241 aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct atataaccta
      301 cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat tttttatgtt
      361 tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca tagagtgaat
      421 cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac aaaatagaag
      481 aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt tctgttcaca
      541 aagtatgcgc aatccacatc ggtatagaat ataatcg
//
