LOCUS       sequence-127236-        7243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Markus M, Du Z, Benezra R
  TITLE     Enhancer-specific modulation of E protein activity.
  JOURNAL   J Biol Chem. 2002 Feb 22. 277(8):6469-77.
  PUBMED    11724804
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Feb 22. 277(8):6469-77."
COMMENT     Sequence Label: MCK-luciferase
FEATURES             Location/Qualifiers
     source          1..7243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3443..5095
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3509..3527)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5166..5300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5203..5222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5257..5276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5494..6082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5574..5593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6253..7044,7045..7113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6876..6895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7114..7218)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccctcgaag cggccgccgc ggcctcgacc aggtacctgg aagcttgcat gcctgcaggt
       61 cgactctaga attccccatc ctggtctata gagagagttc cagaacagcc agggctacag
      121 ataaacccat ctggaaaaac aaagttgaat gacccaagag gggttctcag agggtggcgt
      181 gtgctccctg gcaagcctat gacatggccg gggcctgcct ctctctgcct ctgaccctca
      241 gtggctccca tgaactcctt gcccaatggc atctttttcc tgcgctcctt gggttattcc
      301 agtctcccct cagcattcct tcctcagggc ctcgctcttc tctctgctcc ctccttgcac
      361 agctggctct gtccacctca gatgtcacag tgctctctca gaggaggaag gcaccatgta
      421 ccctctgttt cccaggtaag ggttcaattt ttaaaaatgg ttttttgttt gtttgtttgt
      481 ttgtttgttt gtttgttttt caagacaggg ctcctctgtg tagtcctaac tgtcttgaaa
      541 ctccctctgt agaccaggtc gacctcgaac tcttgaaacc tgccacggac cacccagtca
      601 ggtatggagg tccctggaat gagcgtcctc gaagctaggt gggtaagggt tcggcggtga
      661 caaacagaaa caaacacaga ggcagtttga atctgagtgt attttgcagc tctcaagcag
      721 gggattttat acataaaaaa aaaaaaaaaa aaaaaaccaa acattacatc tcttagaaac
      781 tatatccaat gaaacaatca cagataccaa ccaaaaccat tgggcagagt aaagcacaaa
      841 aatcatccaa gcattacaac tctgaaacca tgtattcagt gaatcacaaa cagaacaggt
      901 aacatcatta ttaatataaa tcaccaaaat ataacaattc taaaaggatg tatccagtgg
      961 gggctgtcgt ccaaggctag tggcagattt ccaggagcag gttagtaaat cttaaccact
     1021 gaactaactc tccagcccca tggtcaatta ttatttagca tctagtgcct aatttttttt
     1081 tataaatctt cactatgtaa tttaaaacta ttttaattct tcctaattaa ggctttcttt
     1141 accatatacc aaaattcacc tccaatgaca cacgcgtagc catatgaaat tttattgttg
     1201 ggaaaatttg tacctatcat aatagttttg taaatgattt aaaaagcaaa gtgttagccg
     1261 ggcgtggtgg cacacgcctt taatccctgc actcgggagg caggggcagg aggatttctg
     1321 agtttgaggc cagcctggtc tacagagtga gttccaggac agccagggct acacagagaa
     1381 accctgtctc gaacccccca ccccccaaaa aaagcaaagt gttggtttcc ttggggataa
     1441 agtcatgtta gtggcccatc tctaggccca tctcacccat tattctcgct taagatcttg
     1501 gcctaggcta ccaggaacat gtaaataaga aaaggaataa gagaaaacaa aacagagaga
     1561 ttgccatgag aactacggct caatattttt tctctccggc gaagagttcc acaaccatct
     1621 ccaggaggcc tccacgtttt gaggtcaatg gcctcagtct gtggaacttg tcacacagat
     1681 cttactggag gtggtgtggc agaaacccat tccttttagt gtcttgggct aaaagtaaaa
     1741 ggcccagagg aggcctttgc tcatctgacc atgctgacaa ggaacacggg tgccaggaca
     1801 gaggctggac cccaggaaca ccttaaacac ttcttccctt ctccgccccc tagagcaggc
     1861 tcccctcacc agcctgggca gaaatggggg aagatggagt gaagccatac tggctactcc
     1921 agaatcaaca gagggagccg ggggcaatac tggagaagct ggtctccccc caggggcaat
     1981 cctggcacct cccaggcaga agaggaaact tccacagtgc atctcacttc catgaatccc
     2041 ctcctcggac tctgaggtcc ttggtcacag ctgaggtgca aaaggctcct gtcatattgt
     2101 gtcctgctct ggtctgcctt ccacagcttg ggggccacct agcccacctc tccctaggga
     2161 tgagagcagc cactacgggt ctaggctgcc catgtaagga ggcaaggcct ggggacaccc
     2221 gagatgcctg gttataatta acccagacat gtggctgccc cccccccccc cccaacacct
     2281 gctgcctgag cctcaccccc accccggtgc ctgggtctta ggctctgtac accatggagg
     2341 agaagctcgc tctaaaaata accctgtccc tggtggatcc agggtgaggg gcaggctgag
     2401 ggcggccact tccctcagcc gcaggtttgt tttcccaaga atggtttttc tgcttctgta
     2461 gcttttcctg tcaattctgc catggtggag cagcctgcac tgggcttctg ggagaaacca
     2521 aaccgggttc taacctttca gctacagtta ttgcctttcc tgtagatggg cgactacagc
     2581 cccaccccca cccccgtctc ctgtatcctt cctgggcctg gggatcctag gctttcactg
     2641 gaaatttccc cccaggtgct gtaggctaga gtcacggctc ccaagaacag tgcttgcctg
     2701 gcatgcatgg ttctgaacct ccaactgcaa aaaatgacac ataccttgac ccttggaagg
     2761 ctgaggcagg gggattgcca tgagtgcaaa gccagactgg gtggcatagt tagaccctgt
     2821 ctcaaaaaac caaaaacaat taaataacta aagtcaggca agtaatccta ctcgggagac
     2881 tgaggcagag ggattgttac atgtctgagg ccagcctgga ctacataggg tttcaggcta
     2941 gccctgtcta cagagtaagg ccctatttca aaaacacaaa caaaatggtt ctcccagctg
     3001 ctaatgctca ccaggcaatg aagcctggtg agcattagca atgaaggcaa tgaaggaggg
     3061 tgctggctac aatcaaggct gtgggggact gagggcaggc tgtaacaggc ttgggggcca
     3121 gggcttatac gtgcctggga ctcccaaagt attactgttc catgttcccg gcgaagggcc
     3181 agctgtcccc cgccagctag actcagcact tagtttagga accagtgagc aagtcagccc
     3241 ttggggcagc ccatacaagg ccatggggct gggcaagctg cacgcctggg tccggggtgg
     3301 gcacggtgcc cgggcaacga gctgaaagct catctgctct caggggcccc tccctgggga
     3361 cagcccctcc tggctagtca caccctgtag gctcctctat ataacccagg ggcacagggg
     3421 ctgcccccgg gtcacgatcc aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     3481 attctatcct ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     3541 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta
     3601 cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     3661 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     3721 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     3781 attgctcaac agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt
     3841 gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga
     3901 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     3961 tcccggtttt aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc
     4021 actgataatg aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag
     4081 aactgcctgc gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     4141 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     4201 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     4261 tttacgatcc cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc
     4321 attcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     4381 ttctgggggc gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct
     4441 tccagggata cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     4501 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     4561 tgtggatctg gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag
     4621 aggacctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     4681 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     4741 catagttgac cgcttgaagt ctttaattaa atacaaagga tatcaggtgg cccccgctga
     4801 attggaatcg atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc
     4861 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     4921 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     4981 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     5041 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtaaaatgt
     5101 aactgtattc agcgatgacg aaattcttag ctattgtaat cctccgaggc gccgtcgaca
     5161 gatctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5281 tatcttatca tgtctggatc gatccggccg cggcggccgc gatccgacgc gaggctggat
     5341 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     5401 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggcc agcaaaaggc
     5461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7201 acaaataggg gttccgcgca catttccccg aaaagtgcca cct
//
