LOCUS       sequence-127256-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target 
            site in the C. elegans genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD162 (Peft-3::Cas9 + Empty sgRNA)
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     89..107
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(145..167)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(439..514)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(516..865)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     871..893
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     885..902
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     886..902
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    910..930
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
       61 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
      121 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
      181 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      241 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgcactga gagtgcacca
      301 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      361 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      421 ccagaaattt cacaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag
      481 ccttatttta acttgctatt tctagctcta aaaccaagac atctcgcaat aggaggtgtt
      541 tatatagggg caacacaaga ggcggcagac atctcgtgag tttgacagag tagtataaag
      601 agtgtgtgtg agagggtgta ggcatttcat aacatatttt ttgtggaata attttggaaa
      661 aaaggtgttt ttttaataat agcttttata agagtttctc aaaaattgca tgcttttttt
      721 gaaaaggttg acaaattata gtagtactaa agttgctatt ttcaggtctc agcagaaaat
      781 tcacaactaa aaataaaatt tgcaaaacag agtgtgcgcc tttaaggtgt acagtttttt
      841 attcctttat tgctagtttt ttttggtttt cccagtcacg acgttgtaaa acgacggcca
      901 gtgaattatc aactatgtat aataaagttg tgagactttt t
//
