LOCUS       sequence-127257-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     pBabe-IRES-EGFP plasmids
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe puro IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     186..208
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     224..240
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(501..518)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     728..747
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
       61 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
      121 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
      181 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
      241 tccttctcta ggcgccggcc ggatccgggc tcgagcccga attcgggaga tctcccatcg
      301 atggggtcga cggtaccgcg ggcccgggat ccgcccctct ccctcccccc cccctaacgt
      361 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
      421 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
      481 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
      541 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
      601 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
      661 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
      721 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
      781 ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag
      841 gttaaaaaaa
//
