LOCUS       sequence-127260-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TCR beta chain genomic transgenic construct for creating OT-1 mice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Carbone FR, Sterry SJ, Butler J, Rodda S, Moore MW
  TITLE     T cell receptor alpha-chain pairing determines the specificity of 
            residue 262 within the Kb-restricted, ovalbumin257-264 determinant.
  JOURNAL   Int Immunol. 1992 Aug;4(8):861-7.
  PUBMED    1419956
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int 
            Immunol."; date: "1992-08"; volume: "4(8)"; pages: "861-7"
COMMENT     Sequence Label: pKS913.CB18.31
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     59..75
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     59..75
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
       61 tagaactagt ggatctgcat ggtgcatact gaaggcttct gtttcctgtg tgacttctaa
      121 cagatgtaag atagtttaga tggggggctt gagatgagat atatgggttc ccctttagag
      181 accctcctct tggttgttct cactgtcacg gtgatagcta gaggctgagg tagaaagggc
      241 tgcatgagaa gggtttgaag acaggatgtg gcaagtgtgg ttcccaaaat gctcagaatg
      301 tgagtgagtg ggggagcatt cacaggcacc ccagatgtag gacctggtaa atgtcaaacc
      361 acatcctgtt gtgactcctg gagatggagc tatggcaggt ggccattctt acttggtgct
      421 gatgttactt acagataaac agccagacct ggggtgagat gcttccaccc cttcccccat
      481 tgtcttcagg aaccgaaaag tcccaggaat gcatctcttc acttgctgat gtttgatgaa
      541 aaacagttct tcttaagatt atttttttta tcatgttcct atatctcttt gctggaatcg
      601 gtcagggaaa caagtattaa gggtcctact gtgcctggtt aactatggga ctcaagatca
      661 gcactgtccc tttgcaggtc agatgccttg taagatatat cacaatgtgt gtgtctggag
      721 ctgggaagga cttagatttc cccacttacc attctgtcag tgctacaagg cctgcagaat
      781 tttcatcaac aaaccactaa gtagggtctg aggctcctta tggagcacct tggttcacag
      841 gaattgaagg gatctggaaa gctggagatg gtaaatgcat tctgcagctg gggagagagg
      901 agggggaaga actgattctc tgagttttag cagatcctgg actccaggca gagtttggag
      961 aacatgtgct ccatatcgag ctgagatcca gatcccccgg gctgcaggaa ttcgatatca
     1021 agcttatcga taccgtcgac ctgcaggtca acggatcttc tacctcaatg ttgttaccac
     1081 ttgta
//
