LOCUS       sequence-127263-       12700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to label reporter mRNAs. Drosophila expression of the MCP 
            protein fused to TagRFP-T..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 12700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pHsp83-NLS-HA-2xMCP-2xTagRFP-T
FEATURES             Location/Qualifiers
     source          1..12700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1495..1696,1697..1727)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(2266..2285)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2385..2407
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2445..2463)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2531..2635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2636..2704,2705..3496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2854..2873)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3667..4255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4156..4175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(4490..4520,4521..5075)
                     /label=P element 5' end
     primer_bind     9161..9179
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(10218..10234)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10219..10235)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             10300..10320
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10324..10350
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             10324..10350
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10351..10362
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12667..12687)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtacctctag agtcgacctc gaacgttaac gttaacgtaa cgttaactcg aggagcttga
       61 taacattata cctaaaccca tggtcaagag taaacatttc tgcctttgaa gttgagaaca
      121 caattaagca tcccctggtt aaacctgaca ttcatacttg ttaatagcgc cataaacata
      181 gcaccaattc gaagaatact tgttaatagc gccataaaca tagcaccaat ttcgaagaaa
      241 ctcagttaaa agcaattagc aattagcaat tagcaataac tctgctgact tcaaaacgag
      301 aagagttgca agtatttgta aggcacagtt tatagaccac cgacggctca ttagggctcg
      361 tcatgtaact aagcgcggtg aaacccaatt gaacatatag tggaattatt attatcaatg
      421 gggaagattt aaccctcagg tagcaaagta atttaattgc aaatagagag tcctaagact
      481 aaataatata tttaaaaatc tggccctttg accttgcttg tcaggtgcat ttgggttcaa
      541 tcgtaagttg cttctatata aacactttcc ccatccccgc aataatgaag aataccgcag
      601 aataaagaga gatttgcaac aaaaaataaa ggcattgcga aaacttttta tgggggatca
      661 ttacactcgg gcctacggtt acaattccca gccacttaag cgacaagttt ggccaacaat
      721 ccatctaata gctaatagcg caatcactgg taatcgcaag agtatatagg caatagaacc
      781 catggatttg accaaaggta accgagacaa tggagaagca agaggatttc aaactgaaca
      841 cccacagtgc tgtgtactac cactggcgcg tttgggagct cactggcctg atgcgtcctc
      901 cgggcgtttc aagcctgctt tacgtggtat actccattac ggtcaacttg gtggtcaccg
      961 tgctgtttcc cttgagcttg ctggccaggc tgctgttcac caccaacatg gccggattgt
     1021 gcgagaacct gaccataact attaccgata ttgtggccaa tttgaagttt gcgaatgtgt
     1081 acatggtgag gaagcagctc catgagattc gctctctcct aaggctcatg gacgctagag
     1141 cccggctggt gggcgatccc gaggagattt ctgccttgag gaaggaagtg aatatcgcac
     1201 agggcacttt ccgcaccttt gccagtattt tcgtatttgg cactactttg agttgcgtcc
     1261 gcgtggtcgt tcgcccggat cgagagctcc tgtatccggc ctggttcggc gttgactgga
     1321 tgcactccac cagaaactat gtgctcatca atatctacca gctcttcggc ttgatagtgc
     1381 aggctataca gaactgcgct agtgactcct atccgcctgc gtttctctgc ctgctcacgg
     1441 gtcatatgcg tgctttggag ctgagggtgc ggcggattgg ctgcagccaa gctttgcgta
     1501 ctcgcaaatt attaaaaata aaactttaaa aataatttcg tctaattaat attatgagtt
     1561 aattcaaacc ccacggacat gctaagggtt aatcaacaat catatcgctg tctcactcag
     1621 actcaatacg acactcagaa tactattcct ttcactcgca cttattgcaa gcatacgtta
     1681 agtggatgtc tcttgccgac gggaccacct tatgttattt catcatggtc tggccattct
     1741 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     1801 gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt
     1861 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
     1921 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
     1981 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
     2041 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
     2101 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
     2161 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
     2221 gcgattcggg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2281 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2341 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2401 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     2461 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     2521 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2581 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2641 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2701 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2761 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2821 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2881 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2941 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3001 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3061 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3121 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3181 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3241 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3301 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3361 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3421 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3481 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3541 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3601 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3661 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3721 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3781 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3841 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3901 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3961 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4021 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4081 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4141 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4201 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4261 cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg
     4321 gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga
     4381 aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac
     4441 gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggccc atgatgaaat
     4501 aacataaggt ggtcccgtcg atagccgaag cttaccgaag tatacactta aattcagtgc
     4561 acgtttgctt gttgagagga aaggttgtgt gcggacgaat ttttttttga aaacattaac
     4621 ccttacgtgg aataaaaaaa aatgaaatat tgcaaatttt gctgcaaagc tgtgactgga
     4681 gtaaaattaa ttcacgtgcc gaagtgtgct attaagagaa aattgtggga gcagagcctt
     4741 gggtgcagcc ttggtgaaaa ctcccaaatt tgtgataccc actttaatga ttcgcagtgg
     4801 aaggctgcac ctgcaaaagg tcagacattt aaaaggaggc gactcaacgc agatgccgta
     4861 cctagtaaag tgatagagcc tgaaccagaa aagataaaag aaggctatac cagtgggagt
     4921 acacaaacag agtaagtttg aatagtaaaa aaaatcattt atgtaaacaa taacgtgact
     4981 gtgcgttagg tcctgttcat tgtttaatga aaataagagc ttgagggaaa aaattcgtac
     5041 tttggagtac gaaatgcgtc gtttagagca gcagccgaat taattctagt tccagtgaaa
     5101 tccaagcatt ttctaaatta aatgtattct tattattata gttgttattt ttgatatata
     5161 taaacaacac tattatgccc accatttttt tgagatgcat ctacacaagg aacaaacact
     5221 ggatgtcact ttcagttcaa attgtaacgc taatcactcc gaacaggtca caaaaaatta
     5281 ccttaaaaag tcataatatt aaattagaat aaatatagct gtgagggaaa tatatacaaa
     5341 tatattggag caaataaatt gtacatacaa atatttatta ctaatttcta ttgagacgaa
     5401 atgaaccact cggaaccatt tgagcgaacc gaatcgcgcg gaactaacga cagtcgctcc
     5461 aaggtcgtcg aacaaaaggt gaatgtgttg cggagagcgg gtgggagaca gcgaaagagc
     5521 aactacgaaa cgtggtgtgg tggaggtgaa ttatgaagag ggcgcgcgat ttgaaaagta
     5581 tgtatataaa aaatatatcc cggtgtttta tgtagcgata aacgagtttt tgatgtaagg
     5641 tatgcaggtg tgtaagtctt ttggttagaa gacaaatcca aagtctactt gtggggatgt
     5701 tcgaagggga aatacttgta ttctataggt catatcttgt ttttattggc acaaatataa
     5761 ttacattagc tttttgaggg ggcaataaac agtaaacacg atggtaataa tggtaaaaaa
     5821 aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg cccgaagtct
     5881 tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac ccacgtagtc
     5941 cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc ccgaactggg
     6001 gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg cccattggtt
     6061 aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc atttgaggta
     6121 tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg gtatgataac
     6181 cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata ttagatatcc
     6241 gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga agaagtgcag
     6301 cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca gtggcactgt
     6361 gagaaaaagc ggcaattcgg caatcgtttt gcccagaaag tatgtgtcac agcgataaag
     6421 tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct aaatcgaatc
     6481 gattcattag aaagttagta aattattgaa atgcaaatgt attctaaaca tgacttacat
     6541 ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag atggctccgt
     6601 tgatattcat cacacccact tgcgtgagtt gttggcccaa aaagatgagg ccaatcaaga
     6661 tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcgc gagttaaatg
     6721 aaatttattt atcttctgca aaactataaa ctatacatct cattgaaaaa aactaagaag
     6781 ggtgtggaat caggcaattc tatctaaaat ctagcgaatt tgtttccaag aattgtaagc
     6841 gttatatcat ttgtttccac tggaaccact caccgttgtc tgaataagtc gcacttttac
     6901 gaggagtggt tccttgagca ccgacagcca ggatcgccac aggaccgccc ggaactgcat
     6961 gaaccaggtg gccttgtagg tgtacccatt ctccggctgc tccagtggct tctccagatt
     7021 tttggtggcc aacaactgct ccatatcccg ggctactttg ctaatggcaa aattgtcgca
     7081 tatcttggcg atccgatcac gggactcgat ctcccgtccg ggcacaacgg ccaacacctg
     7141 tacgtaaaag tccgccggat tgtagttggt aggacactgg gcacccacgc tggataggag
     7201 ttgagatgcc cttaataaac acatcgaact cactaggaaa agaagtcgac ggcttcgctg
     7261 ggagtgccca agaaagctac cctgccctcg gccatcagaa ggatcttgtc aaagagctca
     7321 aacagctcgg aagacggctg atgaatggtc aggatgacgg tcttgccctt ctgcgacagc
     7381 ttcttcagca cctggacgac gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca
     7441 tcgcagatca gaagcggcgg atcggttaga gcctcggagg cgaatgccag acgcttcctt
     7501 tctccgccgg acagaccttt caccctgccg ggcacaccga tgatcgtgtg ctgacatttg
     7561 ctgagcgaaa gctcctggat cacctgatcc acgcgggcca ctcgctgccg ataggtcaga
     7621 tgtcgtggca tccgcaccat ggcttggaaa atcaggtgtt ccctggccgt tagggagccg
     7681 ataaagaggt catcctgctg gacataggcg cacctggcct gcatctcctt ggcgtccaca
     7741 ggttggccat tgagcagtcg catcccggat ggcgatactt ggatgccctg cggcgatcga
     7801 aaggcaaggg cattcagcag ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa
     7861 agttcgcccg gataggccac gccgcaaact gagtttcaaa ttggtaattg gaccctttat
     7921 taagatttca cacagatcag ccgactgcga atagaaactc accgttcttg agcaaatgtt
     7981 tcctgggcgc cggtatgtgt cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct
     8041 gccgccatcc ggagcccggc tgattgaccg ccccaaagat gtccatattg tgccaggcat
     8101 aggtgaggtt ctcggctagt tggccgctcc ctgaaccgga gtcctccggc ggactgggtg
     8161 gccggagcgt gccgtagttt ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag
     8221 ccgctccgct gtcaccctgc aatgataggg gatctcaaat atcaactaca agcgttatgc
     8281 tcatctaacc ccgaacaaaa agtaccccga agtatcctac gaagtaggtt tatactttta
     8341 tttatttttt gtgcatctag gatcagctta aaatatctgg ttgttatatt ttttgtaaaa
     8401 aagaatatag tcgaaaatga atgcctttag atgtcttgat catgatatga tctcaaaaat
     8461 tgtcttatat agcgagaaca gctaccagaa taatctgttt cgtgtcacta tttgtttgtg
     8521 caattgcggt ttgggatttt tgtgggtcgc agttctcacg ccgcagacaa tttgatgttg
     8581 caatcgcagt tcctatagat caagtgaact taagatgtat gcacatgtac tactcacatt
     8641 gttcagatgc tcggcagatg ggtgtttgct gcctccgcga attaatagct cctgatcctc
     8701 ttggcccatt gccgggattt ttcacacttt cccctgctta cccacccaaa accaatcacc
     8761 accccaatca ctcaaaaaac aaacaaaaat aagaagcgag aggagttttg gcacagcact
     8821 ttgtgtttaa ttgatggcgt aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg
     8881 caactgaagg cggacattga cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg
     8941 acgcagcggc gaaagagacg gcgatatttc tgtggacaga gaaggaggca aacagcgctg
     9001 actttgagtg gaatgtcatt ttgagtgaga ggtaatcgaa agaacctggt acatcaaata
     9061 cccttggatc gaagtaaatt taaaactgat cagataagtt caatgatatc cagtgcagta
     9121 aaaaaaaaaa atgttttttt tatctacttt ccgcaaaaat gggttttatt aacttacata
     9181 catactagaa ttggccgctc tagccccccc tcgaatgttc tctctcttct cttctctctc
     9241 tctttctcga atgttctctc tcttctcttc tctctctctt tctcgaggtc atcaagctta
     9301 ggagatcttt aacggggaac ttgaagaagt gcatattggg gttgcggcta gaacccaccg
     9361 gacaatcacg aaaacaacac ttagtgccgc ccatttgttt aaatataagc aaacaacttt
     9421 tatgttatta gtggtggaag tgttagcgtc agctggtgat atcgatggga ggcatcgata
     9481 acagaattga ccgaaaccaa atgatcgata tgacacttct taattaatga gagatttttt
     9541 acttgactgg gcttgtagca ggttttgcac agaagcaatt attttccgga atgtgaaatg
     9601 tctgcttttt agctaattac aacaaaaact ttccaatttt tgttccccaa acccactcaa
     9661 gtgatttcaa attttaccgt ccgcttaaaa tggaactagt ttattccaga ggaaccagct
     9721 tgcaccacca agtctctgaa actctggaaa tatcgatagt ctggtggaga aaagtattca
     9781 taaatataaa taaaaattaa caggtcataa gctgatttgt ttattattta ctgttaaaac
     9841 aagtaaaata atattgggaa caattaaatt ttccattttc ctaattacag tataagccta
     9901 gtgggcgttt tgatatccaa ttgtaatgtt ttaagcaatc ccagtgggct ttgctcaatc
     9961 gttcggacca cttagacgaa tttccaccaa acttagttca gtataatttt tgaattcgcc
    10021 cgcacaggtt gcgcactttt cgaccgtatc acaacactga tctaccctag tattcacagg
    10081 aagttgcatc cctggcatcc agaagcctct agaagtttct agagacttcc agttcgggtc
    10141 gggtttttct ataaaagcag acgcgcggcg tttgccggtt cgagtcttga aaaaaatttc
    10201 gtatcaagct agcttatcga taccgtcgac ctcgaggggg ggcccggtac atcgaattcc
    10261 tgcagcccgg gggatcgatc caccgcggcc gcaatgggcc caaaaaagaa aagaaaagtt
    10321 ggctacccct acgacgtgcc cgactacgcc atcgaaggcc gccatatgct agccgttaaa
    10381 atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg cgacgtgact
    10441 gtcgccccaa gcaacttcgc taacgggatc gctgaatgga tcagctctaa ctcgcgttca
    10501 caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg caaatacacc
    10561 atcaaagtcg aggtgcctaa aggcgcctgg cgttcgtact taaatatgga actaaccatt
    10621 ccaattttcg ccacgaattc cgactgcgag cttattgtta aggcaatgca aggtctccta
    10681 aaagatggaa acccgattcc ctcagcaatc gcagcaaact ccggcatcta cgccatggct
    10741 tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt gactgtcgcc
    10801 ccaagcaact tcgctaacgg gatcgctgaa tggatcagct ctaactcgcg ttcacaggct
    10861 tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata caccatcaaa
    10921 gtcgaggtgc ctaaaggcgc ctggcgttcg tacttaaata tggaactaac cattccaatt
    10981 ttcgccacga attccgactg cgagcttatt gttaaggcaa tgcaaggtct cctaaaagat
    11041 ggaaacccga ttccctcagc aatcgcagca aactccggca tctacgcgga ttctagagtg
    11101 agcaagggcg aggagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
    11161 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
    11221 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
    11281 gctaccagct tcatgtacgg cagcagaacc ttcatcaacc acacccaggg catccccgac
    11341 ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
    11401 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
    11461 gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
    11521 ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgga aggcagaacc
    11581 gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttcaa gaccacatac
    11641 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt ggaccacaga
    11701 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga ggtggctgtg
    11761 gccagatact gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg
    11821 tacaagaccg gttcaggttc atcaggcaca gctagttcaa ctagaatggt gagcaagggc
    11881 gaggagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
    11941 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
    12001 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
    12061 ttcatgtacg gcagcagaac cttcatcaac cacacccagg gcatccccga cttctttaag
    12121 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
    12181 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
    12241 agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag
    12301 gccaacaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaac cgacatggcc
    12361 ctgaagctcg tgggcggggg ccacctgatc tgcaacttca agaccacata cagatccaag
    12421 aaacccgcta agaacctcaa gatgcccggc gtctactatg tggaccacag actggaaaga
    12481 atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac
    12541 tgcgacctcc ctagcaaact ggggcacaaa cttaatggca tggacgagct gtacaagtaa
    12601 atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    12661 taactatgtt gctcctttta cgctatgtgg atacgctgcg
//
