LOCUS       sequence-127264-        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to label reporter mRNAs. Drosophila expression of the MCP 
            protein fused to TagRFP-T..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pHsp83-NLS-HA-2xMCP-2xTagRFP-T
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..36
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             10..36
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             37..48
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agagttggct acccctacga cgtgcccgac tacgccatcg aaggccgcca tatgctagcc
       61 gttaaaatgg cttctaactt tactcagttc gttctcgtcg acaatggcgg aactggcgac
      121 gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg aatggatcag ctctaactcg
      181 cgttcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa
      241 tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt cgtacttaaa tatggaacta
      301 accattccaa ttttcgccac gaattccgac tgcgagctta ttgttaaggc aatgcaaggt
      361 ctcctaaaag atggaaaccc gattccctca gcaatcgcag caaactccgg catctacgcc
      421 atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg cgacgtgact
      481 gtcgccccaa gcaacttcgc taacgggatc gctgaatgga tcagctctaa ctcgcgttca
      541 caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg caaatacacc
      601 atcaaagtcg aggtgcctaa aggcgcctgg cgttcgtact taaatatgga actaaccatt
      661 ccaattttcg ccacgaattc cgactgcgag cttattgtta aggcaatgca aggtctccta
      721 aaagatggaa acccgattcc ctcagcaatc gcagcaaact ccggcatcta cgcggattct
      781 agagtgagca agggcgagga gctgattaag gagaacatgc acatgaagct gtacatggag
      841 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      901 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      961 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaaccacac ccagggcatc
     1021 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggaagnnag tcaccacata
     1081 cgaanaacgg gggcgtgctg accgctaccc aggannccag cctccaggan ggnngcctca
     1141 tctacaacgt caagnnncna aaggggtgaa cttcccatcc aannggccct
//
