LOCUS       sequence-127267-       15500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  oskar translation biosensor with 12x copies of modified PP7 stem 
            loops and 6xMS2 stem loops. . full genomic region, used for P 
            element-mediated germline transformation in Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15500)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 15500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pCasper4-g-osk-TRICK
FEATURES             Location/Qualifiers
     source          1..15500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        5462..5480
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5501..5519
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5571..5589
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5610..5628
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5680..5698
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        5719..5737
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     gene            7776..11911
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(7785..7803)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(11922..12476,12477..12507)
                     /label=P element 5' end
     rep_origin      complement(12742..13330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12822..12841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13501..14292,14293..14361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14124..14143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14362..14466)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14534..14552
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14590..14612)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14712..14731
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(15268..15298,15299..15500)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttgggc tgcaggtcga cctcgagtgt tttgccattt tacaatctgt tggccaacct
       61 gtaaccggtc atcataaagg agttagccat gcactgtcca aaaattgatt gtagaccaag
      121 aacttaatct cgaattaagt taacttcata ttccaatcat tccaaactat tacaaatcga
      181 aatggattga ttgactagaa cgatctttcc gtatatttta tttattttcc gttttatttt
      241 tccgtttttt tcttcagtgt gttccacatc catagaatta cagcagggct ctaattacgt
      301 gtgtttgaat tgcggcgccc tgattaaggc ccttttgcag ggcaatcaat cgatttgctg
      361 cggagtaata ataacagatg ttaagccaga ctctggaaac ggataaggat aattactgga
      421 aattagtgat ggagctgccg ccgaaaggcg atcagattca gttgccattg gggtgaactt
      481 ctggttaatc gtgtgcaacg caatcagcat tcctgagatt ttccgcctgt cagcagatta
      541 acaggtgtct ctccgcccca ccaaagtgag gcaatagcta aattgttcgc ataaattgat
      601 tacaataatt tgcctatccc ccaacattta gccgccctct gcgaaatgaa ttcgctgctg
      661 gtaaaaattc taagacagca gacagaataa caacaagttg ctccgtcggc ttccataatt
      721 tatgcagaaa tgcagtcgaa cccaatgaaa agtgtcgttc acaaatctag gcggtaaatt
      781 gctttttgcg atttccaatg cacacccacc acacgcaaat gcaaaagttg tcaatcaaaa
      841 atggtcgtca atcatggtag tagttgttag aaaggaagag agtaggaaaa tgtctttaaa
      901 aatattaaac aaatttattt ttcagtggtt acttcctaca attacgctta tagaaataca
      961 aggaaggttg ccaaatacca taaatatatg tataattcct taccttacaa tcgagtgacc
     1021 actgtaattc ttagctggga gtagagagaa tgagagaggg cctggaatgc aaattactat
     1081 tattaccgga caattgccct gtgctgtgcg agtgcattca aaatatttga aacaccaccc
     1141 gaaaagggaa aattgaatgg caagaaaagc cggctgattc atcccactct aaattattgg
     1201 gcttacacat tcagcaaata aaaatgggag aagggtcgag atttataaat tggaccacat
     1261 gttctcgccc agtttttcta catacatata gtattgtatt gctcgtcacg tctctgcagc
     1321 atcctctgca attaaggcaa ggctagggaa aatatacagt ccaacatcta taaggaagta
     1381 cttccctaaa ttacagatag gttgtagtgg cactaactat tgcccattct atatatatta
     1441 ccctttgaac ccatcaacac cttattgaaa attatttttt tattattctg atctgctagt
     1501 taagtaaaat ttttactgat ttaatttttg taatttcttg ctggtacgca ttactgatgt
     1561 ttgactgtat ccaacttgct taagctaaga agccataatc ctccttccga ttgtctgtgc
     1621 ttcttccggg gtgccggaat catcacgaga tgtaagaaag cccctgagtc atccatcaat
     1681 cgctggacta accctcagag caagtgcctc cattaccctt ctcggagttt caggtgcact
     1741 tgctgtatgg atggataatc atctcctggg gaattgctgc caagcagctc catttgccga
     1801 ttagccgaca gaccaccaga gcaccaccgc acgtggtaac gctcgtcggc accatttgag
     1861 ttattctgga atttatgtat atgctacttc gtaattgctt atcaagcagc tctgcagacc
     1921 cactggcgtg ctgttttcta atctggacca agagaaaggc cattaaaacg aatgtccagc
     1981 tggaacggag tgcaaactgc tttgtgtaat ggtcgtcaaa tggcaatgca ggtggtaatt
     2041 tcgtttaatt gtttttactg gctacgtaga ctgctttgaa tgttaaccta atggtgggcg
     2101 aatgggaaca aattgataaa aaaattaaag aaaatgaata tatatatata tatataggtt
     2161 ctatattagg ttctgaaaac agttttttat tttttcaaaa ttttttggtt gtaccaccct
     2221 aatgtatttt cacaaattct gttgatctgg aaacattatc tctgcaaact gctcaccgtt
     2281 acttctgttg cgcctgctct ttcgccttcc agtacgaaaa gcaccgtaag tctccaaaat
     2341 gcagatgaat ggagaagtgg accgcgttgg cgagggatcc aagaatattg gatcactttc
     2401 ctccaagcga tggccgcagt cacaagtgaa ttccccagca aaccgatcag ttataccagc
     2461 accaatactt ccgccaaaac ctattatctt aagtccgtga aaaagcgggt gaccacgtgt
     2521 ttccagcagt tgcgcgataa actccagtca tccggttcct ttcgcaagag ttcctccagc
     2581 tgcctcaacc agatcttcgt aaggtccgat ttctccgcat gcggcgaacg ctttcggaag
     2641 atcttcaaat cggcgcgaaa aaccgaattg ccggagttgt ggaaggtgcc attggtggcc
     2701 catgaactca ccagccggca gagcagccag cagttgcaag tcgtagccag actcttctcg
     2761 tccactcaga tttccaccaa ggaaatcacc tacaacagca acagcaacac cagcgagaac
     2821 aacatgacca tcatcgagag caactatata tccgtgcgcg aggaatatcc cgatatagat
     2881 agtgaggtgc gcgccatatt gctgagccac gcccagaatg gaatcacgat atcgagcatc
     2941 aagagtgagt agctttatgt ttgaaagctt tcttctttcc agaagtttcg acttcatcaa
     3001 acacttcaga actttctcta agtggctata tttagttaga aatctaaaat gtataaattt
     3061 ttagtaggtt attaaatttt atttggatta tataactttt atagtttagt atatttattt
     3121 taatttatat tttttctatg tttctttttt gtaagattac ccttaaataa ttctataatt
     3181 gaaattcgcc aggtgaatat cgaaaactga cgggcaatcc atttccactg cacgacaacg
     3241 tgacggattt cctgctgacc attcccaatg tgaccgctga gtgcagcgag tccggtaagc
     3301 ggatcttcaa cctgaaagcg agcctgaaga acggtcacct cctggatatg gtgctcaacc
     3361 agaaagagcg caccagcgac tacagcagcg gagctccgtc cctggagaac ataccacgag
     3421 cacctccacg ctactggaag aatcccttca aacggagggc tctgtcccag ctgaacacca
     3481 gcccgaggac cgtgcccaag ataacggatg aaaagaccaa ggatatcgcc accaggccgg
     3541 tttcgctgca tcaaatggcc aatgaggcag cggagtcgaa ctggtgctac caggataatt
     3601 ggaagcatct gtgagtactg aaaaggtgtt ttgtagacag tgcaactata gcaaaaataa
     3661 acctttactt aacaaaatgt actaatcttt ctactgaact cctagcaaca atttctacca
     3721 gcaagccagc gtaaatgcgc caaaaatgcc agtacccatc aacatctaca gccccgatgc
     3781 cccagaggaa ccaatcaatt tggctccacc tgggcatcag ccaagctgca gaacccaaag
     3841 ccagaaaacc gaaccgactg aaaaccgcca tttgggcatc tttgtgcatc catttaacgg
     3901 catgaacata atgaagagac gccacgaaat gacgcccacg ccaacgattt taaccagtgg
     3961 aacctacaac gattctctgc tgacgattaa ctcggattac gatgcctatc tgctggactt
     4021 tccgcttatg ggcgatgatt ttatgctata tctcgcccga atggagctaa aatgccgatt
     4081 taggcgtcac gaacgcgtca gatcctacct gcaaagcacc agaggatatg gcctcggtgc
     4141 ctcagcatct tcagatgtgc tgtcactgtc caatcttcaa gccagcagag catatgggct
     4201 cgctggctcc actgtcatta cagtggacct gatcttctcc aatcaaagga gcagaggata
     4261 tggcctcgct ccctccgact acatcaggct ggaagtgaca tccatcattc atcccagcag
     4321 agcatatggg ctcgctggga ccacagcatg cctcgaagtg ctgagagact atcatcaaac
     4381 cgacagagga tatggcctcg tcggctccta ctccctcctg gtggaatact tctccactct
     4441 tcaacccagc agagcatatg ggctcgctgg gtccatcaga gatacaagat ctctcgacag
     4501 atcctacctg caaagcacca gaggatatgg cctcggtgcc tcagcatctt cagatgtgct
     4561 gtcactgtcc aatcttcaag ccagcagagc atatgggctc gctggctcca ctgtcattac
     4621 agtggacctg atcttctcca atcaaaggag cagaggatat ggcctcgctc cctccgacta
     4681 catcaggctg gaagtgacat ccatcattca tcccagcaga gcatatgggc tcgctgggac
     4741 cacagcatgc ctcgaagtgc tgagagacta tcatcaaacc gacagaggat atggcctcgt
     4801 cggctcctac tccctcctgg tggaatactt ctccactctt caacccagca gagcatatgg
     4861 gctcgctggg tccatcagag atacaagatc ttcacgcgtc ctgcagtcag gactttgtgt
     4921 atccggactg acgatcaatg gcgcccgaaa tcgtttaaaa agagtccaat tacccgaggg
     4981 tactcagatc atcgtcaata tcggatcggt ggacattatg cgcggcaagc ctttggttca
     5041 gatcgagcac gattttcggc tactgatcaa ggagatgcac aatatgcgat tggtgccgat
     5101 tctaacaaat cttgcaccgc tgggcaacta ttgtcacgat aaggtattat gtgacaaaat
     5161 ctaccgattc aacaagttta tccgaagcga atgctgtcac ctaaaggtca tcgacataca
     5221 ctcctgtcta atcaacgaaa ggggcgtggt gcgatttgat tgctttcagg cgtaagactt
     5281 tataccatat accatatcat atgagtttac gatttgtgcc acttttcagc tcaccacgcc
     5341 aagtcaccgg ttccaaggaa ccctatctgt tctggaacaa aatcggtcgg cagcgcgtac
     5401 tgcaagttat tgaaacgagt ctggagtatt aaggatccta aggtacctaa ttgcctagaa
     5461 aacatgagga tcacccatgt ctgcaggtcg actctagaaa acatgaggat cacccatgtc
     5521 tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa acatgaggat
     5581 cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct gcagtattcc
     5641 cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc acccatgtct
     5701 gcaggtcgac tctagaaaac atgaggatca cccatgtctg cagtattccc gggttcatta
     5761 gatccttggg ttcttaatca agatacatat atgcaaattt tgactgggct ggcaccggaa
     5821 ccgacaaaat aagaactttt tgatgatttt acgatttacg ctgatggatc gctgctttta
     5881 cggaattcgc ttagttttaa tatgttttat atgtagtatg ttctctgtct ttgtttattt
     5941 atatgttcgt gcacttgtcc tagtccatta ttgtatatta ttgtgtgttt tgtgttctat
     6001 gttagattta aacttctcaa tttttcgctg tctgtgattt gttttgccaa tgccattgat
     6061 tttctgcaca ctttttgctg ctatcccaaa agctgtgtaa aataatcaaa tgcaaaataa
     6121 gcgcaagcag ctgaaaactt ctcttcaaac ttttccgctt ttcccaaaac cattttgctt
     6181 tgaaatctgt ttttaccaaa tcagattaaa ctgcaaaatg gaacttaaat gcaaatcatt
     6241 gcaatgctta taaactgttt tttgttctat atacttttgt gtgggtcaaa attcggcatg
     6301 ctcctgtatc acacaacctg ccacttgccc ttaaaaagaa gggcgcagtg ggcgtggtac
     6361 gtatacatat gagccatgct gcattttggc cgtaatgaaa atgcactgct ttacttggaa
     6421 aattcgcttg cacaaaatca acgccgcggc tgatttatta ttgatgtgct caagcaaatt
     6481 caagtgaagc atttgcgcga ttttcgtctt tctgtttccg tttgcaaaaa agtttataaa
     6541 atgcttacac tctgctgcag acacgccaac cggaagtgcg cactaagcgc ttgtttgtag
     6601 cacagtgtag aattctggcg taatttacag ctctacttta aagtcttcta gatagctatc
     6661 tactatttat aaacttattt attgtcttga atgtatgtta attgtatgta ttgatggtga
     6721 tcacgttttt tttgtcctat aacaagctgc aatgtaaaat ccaaaaaaaa aatgaaaaaa
     6781 taaaataaaa gggaaatcaa tgattttgtt tgctctaagc tatgttcaaa gtaactggga
     6841 actaatcgaa ctaatttcga tttaaaagtg gtctagatta tattgtgaca tttgtgaata
     6901 tattattata tttgcgaatt tattattatc ataaaaataa cggtagttga aaaataattt
     6961 agatggcatg caatattaaa agttcccgcc aaattggctt gaatctttgc taatatatta
     7021 cagtttccaa ccttgcatta agttcacgat agtttttcgg cattttcgat tgtaaaagca
     7081 taaaaaagtc cctgctatcg aagctctacc acctccatca gcaggcgacg gcaaaaaaat
     7141 cttgaaatgt aagcagtaac gtcaaaattc gctttcaaat cgttaaacgt acgttctcat
     7201 tcgatttcag aacaaaatgg cttcattctt ggcacgaact ggcggccccc tgatcgaaac
     7261 cgttaggcca gcggccataa aaaaggtgag ttcgccaatt agtagtcata tttgtgtgta
     7321 aaaaatgcga aaatccgtcg ctttacataa taaggcgaaa agagagagtg aacacctacg
     7381 ctgtcccctt ctctgtctct cacttactca cacatgtgaa atgctgatcg tttatcggta
     7441 caaaaaaaaa acttaagcga taatttaagt tctttgccat ttgttaacta attgtttctc
     7501 cagagaaacg gggagagtgt gagatttaaa gagagtcacg atcaccaaag acacctgact
     7561 tttttattgt gtcagtgtga cgcaactgtg cgcttttcgt acgcatgtgt gtgtgtgggg
     7621 accttgaaag ccccatttat tcccccactt aatttagttt gttttttatc aatgagcagc
     7681 tgacaactgt tgcttatcgt ttgtggttgt tgttgctgtt gtcgccgggc cctctagatg
     7741 cggccgcgga tctgatcccg ggcgggtacc gaattctagt atgtatgtaa gttaataaaa
     7801 cccttttttg gagaatgtag atttaaaaaa acatattttt tttttatttt ttactgcact
     7861 ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgaag
     7921 taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt
     7981 ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct
     8041 ctttcgccac cgtttgtagc gttacctagc gtcaatgtcc gccttcagtt gcactttgtc
     8101 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     8161 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg
     8221 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
     8281 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt
     8341 agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa
     8401 attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatc gcacaaacaa
     8461 atagtgacac gaaacagatt attctggtag ctgtgctcgc tatataagac aatttttaag
     8521 atcatatcat gatcaagaca tctaaaggca ttcattttcg actacattct tttttacaaa
     8581 aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat
     8641 aaacctactt cgtaggatac ttcgttttgt tcggggttag atgagcataa cgcttgtagt
     8701 tgatatttga gatcccctat cattgcaggg tgacagcgga cgcttcgcag agctgcatta
     8761 accagggctt cgggcaggcc aaaaactacg gcacgctcct gccacccagt ccgccggagg
     8821 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     8881 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     8941 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     9001 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     9061 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     9121 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     9181 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     9241 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     9301 ggaacacctg attttccagg ccatggtgcg gatgccacga catctgacct atcggcagcg
     9361 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     9421 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     9481 cgcctccgag gcactaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     9541 ggactcattt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     9601 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     9661 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     9721 cttttcctag tgagttcgat gtgtttatta agggtatcta gcattacatt acatctcaac
     9781 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     9841 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     9901 ggcgacaatt ttgctattag caaagtagcc cgggatatgg agcagttgtt ggccaccaaa
     9961 aatttggaga agccactgga gcagccggag aatgggtaca cctacaaggc cacctggttc
    10021 atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga accactcctc
    10081 gtaaaagtgc gacttattca gacaacggtg agtggttcca gtggaaacaa atgatataac
    10141 gcttacaatt cttggaaaca aattcgctag attttagtta gaattgcctg attccacacc
    10201 cttcttagtt tttttcaatg agatgtatag tttatagttt tgcagaaaat aaataaattt
    10261 catttaactc gcgaacatgt tgaagatatg aatattaatg agatgcgagt aacattttaa
    10321 tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt
    10381 gggcgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa
    10441 cgtctttgcc acgataaatg taagtcttgt ttagaataca tttgcatatt aataatttac
    10501 taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga
    10561 gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg
    10621 aattaccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg
    10681 gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca
    10741 atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc
    10801 tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact
    10861 cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca
    10921 acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat
    10981 cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg
    11041 ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc
    11101 tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa
    11161 ataactgctt gttttttttt ttaccattat taccatcgtg tttactgttt attgccccct
    11221 caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca
    11281 agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga
    11341 cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat
    11401 atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac
    11461 accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct
    11521 tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg
    11581 gttccgagtg gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt
    11641 tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc taatttaata
    11701 ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga
    11761 actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc
    11821 ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat
    11881 ttagaaaatg cttggatttc actggaacta gaattaattc ggctgctgct ctaaacgacg
    11941 catttcgtac tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga
    12001 acaggaccta acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa
    12061 cttactctgt ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct
    12121 ctatcacttt actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt
    12181 ttgcaggtgc agccttccac tgcgaatctt taaagtgggt atcacaaatt tgggagtttt
    12241 caccaaggct gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca
    12301 cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt
    12361 tttattccac gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct
    12421 ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac
    12481 gggaccacct tatgttattt catcatgggc cagacccacg tagtccagcg gcagatcggc
    12541 ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga
    12601 cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc
    12661 gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc
    12721 cgagttcaag aagaaggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    12781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    12841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    12901 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
    12961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    13021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    13081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    13141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    13201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    13261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    13321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    13381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    13441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    13501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    13561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    13621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    13681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    13741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    13801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    13861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    13921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    13981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    14041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    14101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    14161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    14221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    14281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    14341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    14401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    14461 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    14521 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    14581 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    14641 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    14701 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    14761 accgcaccga atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga
    14821 atgtgttgcg gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg
    14881 gaggtgaatt atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg
    14941 gtgttttatg tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt
    15001 ggttagaaga caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt
    15061 ctataggtca tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg
    15121 caataaacag taaacacgat ggtaataatg gtaaaaaaaa aaacaagcag ttatttcgga
    15181 tatatgtcgg ctactccttg cgtcgggccc gaagtcttag agccagatat gcgagcaccc
    15241 ggaagctcac gatgagaatg gccagaccat gatgaaataa cataaggtgg tcccgtcggc
    15301 aagagacatc cacttaacgt atgcttgcaa taagtgcgag tgaaaggaat agtattctga
    15361 gtgtcgtatt gagtctgagt gagacagcga tatgattgtt gattaaccct tagcatgtcc
    15421 gtggggtttg aattaactca taatattaat tagacgaaat tatttttaaa gttttatttt
    15481 taataatttg cgagtacgca
//
