LOCUS       sequence-127270-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CDS of DsRed as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDsRed (GB0100)
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(60..737)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(122..145)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     537..557
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 cagctccggc cgccatggcg gccgcgggaa ttcgattggg cgatgagtgg tctcgaagcc
       61 tacaggaaca ggtggtggcg gccctcggtg cgctcgtact gctccacgat ggtgtagtcc
      121 tcgttgtggg aggtgatgtc cagcttggcg tccacgtagt agtagccggg cagctgcacg
      181 ggcttcttgg ccatgtagat ggacttgaac tccaccaggt agtggccgcc gtccttcagc
      241 ttcagggcct tgtgtgtctc gcccttcagc acgccgtcgc gggggtacag gcgctcggtg
      301 gaggcctccc agcccatcgt cttcttctgc atcacggggc cgtcggaggg gaagttcacg
      361 ccgatgaact tcaccttgta gatgaagcag ccgtcctgca gggaggagtc ctgggtcacg
      421 gtcgccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag
      481 gacagcttct tgtagtcggg gatgtcggcg gggtgcttca cgtacacctt ggagccgtac
      541 tggaactggg gggacaggat gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc
      601 agcttcacgg tgttgtggcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac
      661 tcgtggccgt tcacggtgcc ctccatgcgc accttgaagc gcatgaactc ggtgatgacg
      721 ttctcggagg aggccattcg agaccactca tcgcccaatc aacta
//
