LOCUS       sequence-127276-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rosa26 targeting vector backbone for use with GMAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: R26TV CAG LSL 2-5
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..230
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    316..349
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    369..503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(393..412)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     447..466
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
       61 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      241 accccggtga acagctcttc gcccttagac accatggtgt ttaaacgatc gcacagctag
      301 cgttcgagtt agcccataac ttcgtataat gtatgctata cgaagttata agcttactta
      361 ccatgtcaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      421 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      481 ttataagctg caataaacaa gttaacaaca acaattgcat tcattctatg tttcaggttc
      541 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      601 attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat
      661 taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct
      721 gtgtggagta agaaaaaaca gt
//
