LOCUS       sequence-127277-       15067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rosa26 targeting vector backbone for use with GMAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15067)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 15067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: R26TV CAG LSL 2-5
FEATURES             Location/Qualifiers
     source          1..15067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..1086
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(1093..1158,1159..1196)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        1210..1589
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1591..1864
                     /label=chicken beta-actin promoter
     intron          1865..2881
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2805..2827
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2889..2908
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(2950..2983)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2990..3038
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3045..3848)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3156..3175)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3766..3785
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     intron          4662..4727
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4857..4877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5302..5436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5339..5358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5393..5412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(5456..5489)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5575..5596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5836..5855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5949..5965)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5984..6208
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    6298..10555
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        10604..11094
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     11027..11046
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11121..11690
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          11767..11832
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    11906..12130
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12222..12242)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12222..12240)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12261..12277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12261..12277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12274..12296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12285..12301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12309..12315,12316..12333,12334..12339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12354..12375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12492..12509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12663..13251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12743..12762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13422..14213,14214..14282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14045..14064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14283..14387)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(14413..14868)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14550..14571)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14762..14781
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14995..15017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15009..15026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15010..15026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15036..15055
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15036..15054
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccccgcggca ggccctccga gcgtggtgga gccgttctgt gagacagccg ggtacgagtc
       61 gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc ggtccgccct gcagcaaccg
      121 gagggggagg gagaagggag cggaaaagtc tccaccggac gcggccatgg ctcggggggg
      181 ggggggcagc ggaggacgct tccggccgac gtctcgtcgc tgattggctt ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctga cgtgcgggcg cggtcggcct ctggcggggc gggggagggg
      421 agggagggtc agcgaaagta gctcgcgcgc gagcggccgc ccaccctccc cttcctctgg
      481 gggagtcgtt ttacccgccg ccggccgggc ctcgtcgtct gattggctct cggggcccag
      541 aaaactggcc cttgccattg gctcgtgttc gtgcaagttg agtccatccg ccggccagcg
      601 ggggcggcga ggaggcgctc ccaggttccg gccctcccct cggccccgcg ccgcagagtc
      661 tggccgcgcg cccctgcgca acgtggcagg aagcgcgcgc tgggggcggg gacgggcagt
      721 agggctgagc ggctgcgggg cgggtgcaag cacgtttccg acttgagttg cctcaagagg
      781 ggcgtgctga gccagacctc catcgcgcac tccggggagt ggagggaagg agcgagggct
      841 cagttgggct gttttggagg caggaagcac ttgctctccc aaagtcgctc tgagttgtta
      901 tcagtaaggg agctgcagtg gagtaggcgg ggagaaggcc gcacccttct ccggaggggg
      961 gaggggagtg ttgcaatacc tttctgggag ttctctgctg cctcctggct tctgaggacc
     1021 gccctgggcc tgggagaatc ccttgccccc tcttcccctc gtgatctgca actccagtct
     1081 ttctagcatc tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg
     1141 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtggtt
     1201 aattaagtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     1261 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1321 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1381 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1441 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1501 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1561 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     1621 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     1681 gtgcagcgat gggggcgggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
     1741 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     1801 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     1861 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     1921 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     1981 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     2041 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     2101 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     2161 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     2221 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     2281 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     2341 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     2401 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     2461 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     2521 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     2581 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     2641 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     2701 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     2761 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2821 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2881 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga
     2941 gttaattaaa taacttcgta tagcatacat tatacgaagt tatggatccg aacaaacgac
     3001 ccaacacccg tgcgttttat tctgtctttt tattgccgat cccctcagaa gaactcgtca
     3061 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     3121 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     3181 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     3241 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     3301 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     3361 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     3421 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     3481 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     3541 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     3601 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     3661 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     3721 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     3781 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatggcc
     3841 gatcccatat tggctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg
     3901 cgaagtggac ctgggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga
     3961 caagacgctg ggcggggttt gctcgacatt gggtggaaac attccaggcc tgggtggaga
     4021 ggctttttgc ttcctcttgc aaaaccacac tgctcgacat tgggtggaaa cattccaggc
     4081 ctgggtggag aggctttttg cttcctcttg caaaaccaca ctgctcgact agtgattaaa
     4141 gtcgactcgg ggacaccaaa tatggcgatc tcggcctttt cgtttcttgg agctgggaca
     4201 tgtttgccat cgatccatct accaccagaa cggccgttag atctgctgcc accgttgttt
     4261 ccaccgaaga aaccaccgtt gccgtaacca ccacgacggt tgttgctaaa gaagctgcca
     4321 ccgccacggc caccgttgta gccgccgttg ttgttattgt agttgctcat gttatttctg
     4381 gcacttcttg gttttcctct taagtgagga ggaacataac cattctcgtt gttgtcgttg
     4441 atgcttaaat tttgcacttg ttcgctcagt tcagccataa tatgaaatgc ttttcttgtt
     4501 gttcttacgg aataccactt gccacctatc accacaacta actttttccc gttcctccat
     4561 ctcttttata ttttttttct cgagggatct ttgtgaagga accttacttc tgtggtgtga
     4621 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     4681 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     4741 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     4801 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     4861 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     4921 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     4981 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     5041 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     5101 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     5161 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     5221 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataga
     5281 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     5341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5401 tccaaactca tcaatgtatc ttatcatgtc tggatctgac atggtaagta agcttataac
     5461 ttcgtatagc atacattata cgaagttatg ggctaactcg aacgctagct gtgcgatcgt
     5521 ttaaacacca tggtgtctaa gggcgaagag ctgttcaccg gggtggtgcc catcctggtc
     5581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     5641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     5701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     5761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     5881 gacaccctgg tgaaccgcat cgagctggtt taaacgttgt tgtttggggt tgaattactc
     5941 tcccggggga tccactagtt ctagagcggc cgatcagcct cgactgtgcc ttctagttgc
     6001 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6061 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6121 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6181 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     6241 agatccacta gttctagcct cgaggctaga gcggccgccg cgccgatatc gaattcgcta
     6301 gaagatgggc gggagtcttc tgggcaggct taaaggctaa cctggtgtgt gggcgttgtc
     6361 ctgcagggga attgaacagg tgtaaaattg gagggacaag acttcccaca gattttcggt
     6421 tttgtcggga agttttttaa taggggcaaa taggaaaatg gaggatagga gtcatctggg
     6481 gtttatgcag caaaactaca ggtatattgc ttgtatccgc ctcggagatt tccatgagga
     6541 gataaagaca tgtcacccga gtttatactc tcctgcttag atcctactac agtatgaaat
     6601 acagtgtgcg aggtagacta tgtaagcaga tttaatcatt ttaaagagcc cagtacttca
     6661 tatccatttc tcccgctcct tctgcagcct tatcaaaagg tatttagaac actcatttta
     6721 gccccatttt catttattat actggcttat ccaaccccta gacagagcat tggcattttc
     6781 cctttcctga tcttagaagt ctgatgactc atgaaaccag acagattagt tacatacacc
     6841 acaaatcgag gctgtagctg gggcctcaac actgcagttc ttttataact ccttagtaca
     6901 ctttttgttg atcctttgcc ttgatcctta attttcagtg tctatcacct ctcccgtcag
     6961 gtggtgttcc acatttgggc ctattctcag tccagggagt tttacaacaa tagatgtatt
     7021 gagaatccaa cctaaagctt aactttccac tcccatgaat gcctctctcc tttttctcca
     7081 ttataactga gctataccat taatggtttc aggtggatgt ctcctccccc aatatacctg
     7141 atgtatctac atattgccag gctgatattt taagacataa aggtatattt cattattgag
     7201 ccacatggta ttgattactg ctactaaaat tttgtcattg tacacatctg taaaaggtgg
     7261 ttccttttgg aatgcaaagt tcaggtgttt gttgtctttc ctgacctaag gtcttgtgag
     7321 cttgtatttt ttctatttaa gcagtgcttt ctcttggact ggcttgactc atggcattct
     7381 acacgttatt gctggtctaa atgtgatttt gccaagcttc ttcaggacct ataattttgc
     7441 ttgacttgta gccaaacaca agtaaaatga ttaagcaaca aatgtatttg tgaagcttgg
     7501 tttttaggtt gttgtgttgt gtgtgcttgt gctctataat aatactatcc aggggctgga
     7561 gaggtggctc ggagttcaag agcacagact gctcttccag aagtcctgag ttcaattccc
     7621 agcaaccaca tggtggctca caaccatctg taatgggatc tgatgccctc ttctggtgtg
     7681 tctgaagacc acaagtgtat tcacattaaa taaataatcc tccttcttct tctttttttt
     7741 tttttaaaga gaatctgtct ccagtagaat tactgaagta atgaaatact ttgtgtttgt
     7801 tccaatatgg agccaataat caaatactct tagcactgga aatgtaccaa ggaactattt
     7861 tatttaagtg actgtggaca gaggagccat aactgcagac ttgtgggata cagaagacca
     7921 atgcagactt aatgtctttt ctcttacact aagcaataaa gaaataaaaa ttgaacttct
     7981 agtatcctat ttgttaaact gctagcttta ctaacttttg tgcttcatct atacaaagct
     8041 gaaagctaag tctgcagcca ttactaaaca tgaaagcaag taatgataat tttggatttc
     8101 aaaaatgtag ggccagagtt tagccagcca gtggtggtgc ttgcctttat gccttaatcc
     8161 cagcactctg gaggcagaga caggcagatc tctgagtttg agcccagcct ggtctacaca
     8221 tcaagttcta tctaggatag ccaggaatac acacagaaac cctgttgggg aggggggctc
     8281 tgagatttca taaaattata attgaagcat tccctaatga gccactatgg atgtggctaa
     8341 atccgtctac ctttctgatg agatttgggt attatttttt ctgtctctgc tgttggttgg
     8401 gtcttttgac actgtgggct ttcttaaagc ctccttccct gccatgtggt ctcttgtttg
     8461 ctactaactt cccatggctt aaatggcatg gctttttgcc ttctaagggc agctgctgag
     8521 tttgcagcct gatttccagg gtggggttgg gaaatctttc aaacactaaa attgtccttt
     8581 aatttttttt taaaaaatgg gttatataat aaacctcata aaatagttat gaggagtgag
     8641 gtggactaat attaatgagt ccctccccta taaaagagct attaaggctt tttgtcttat
     8701 actaactttt tttttaaatg tggtatcttt agaaccaagg gtcttagagt tttagtatac
     8761 agaaactgtt gcatcgctta atcagatttt ctagtttcaa atccagagaa tccaaattct
     8821 tcacagccaa agtcaaatta agaatttctg actttaatgt tatttgctac tgtgaatata
     8881 aaatgatagc ttttcctgag gcagggtctc actatgtatc tctgcctgat ctgcaacaag
     8941 atatgtagac taaagttctg cctgcttttg tctcctgaat actaaggtta aaatgtagta
     9001 atacttttgg aacttgcagg tcagattctt ttatagggga cacactaagg gagcttgggt
     9061 gatagttggt aaatgtgttt aagtgatgaa aacttgaatt attatcaccg caacctactt
     9121 tttaaaaaaa aaagccaggc ctgttagagc atgctaaggg atccctagga cttgctgagc
     9181 acacaagagt agtacttggc aggctcctgg tgagagcata tttcaaaaaa caaggcagac
     9241 aaccaagaaa ctacagtaag gttacctgtc tttaaccatc tgcatataca cagggatatt
     9301 aaaatattcc aaataatatt tcattcaagt tttcccccat caaattggga catggatttc
     9361 tccggtgaat aggcagagtt ggaaactaaa caaatgttgg ttttgtgatt tgtgaaattg
     9421 ttttcaagtg atagttaaag cccatgagat acagaacaaa gctgctattt cgaggtctct
     9481 tggttatact cagaagcact tctttgggtt tccctgcact atcctgatca tgtgctaggc
     9541 ctccttaggc tgattgttgt tcaaataact taagtttcct gtcaggtgat gtcatatgat
     9601 ttcatatatc aaggcaaaac atgttatata tgttaaacat ttgacttaat gtgaaagtta
     9661 ggtctttgtg ggttttgatt ttaatttcaa aacctgagct aaataagtca ttttacatgt
     9721 cttacatttg gtgaattgta tattgtggtt tgcaggcaag actctctgac ctagtaaccc
     9781 tcctatagag cactttgctg ggtcacaagt ctaggagtca agcatttcac cttgaagttg
     9841 agacgttttg ttagtgtata ctagttatat gttggaggac atgtttatcc agaagatatt
     9901 caggactatt tttgactggg ctaaggaatt gattctgatt agcactgtta gtgagcattg
     9961 agtggccttt aggcttgaat tggagtcact tgtatatctc aaataatgct ggcctttttt
    10021 aaaagccctt gttctttatc accctgtttt ctacataatt tttgttcaaa gaaatacttg
    10081 tttggatctc cttttgacaa caatagcatg ttttcaagcc atattttttt tccttttttt
    10141 tttttttttt ggtttttcga gacagggttt ctctgtatag ccctggctgt cctggaactc
    10201 actttgtaga ccaggctggc ctcgaactca gaaatccgcc tgcctctgcc tcctgagtgc
    10261 cgggattaaa ggcgtgcacc accacgcctg gctaagttgg atattttgta tataactata
    10321 accaatacta actccactgg gtggattttt aattcagtca gtagtcttaa gtggtcttta
    10381 ttggccctta ttaaaatcta ctgttcactc taacagaggc tgttggacta gtggactaag
    10441 caacttccta cggatatact agcagataag ggtcagggat agaaactagt ctagcgtttt
    10501 gtatacctac cagcttatac taccttgttc tgatagaaat atttaggaca tctagcttat
    10561 cgatccgtcg acggtatcga taagcttgat atcgaattct accgggtagg ggaggcgctt
    10621 ttccaaggca gtctgagcat gcgcttagca gccccgctgg cacttggcgc tacacaagtg
    10681 gccttggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
    10741 tggccccttc gcgccacctt ctctcctccc ctagtcagga agttcccccc cgccccgcag
    10801 ctcgcgtcgt aggacgtgac aaatggaagt agcacgtctc actagtctcg tcagatggac
    10861 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
    10921 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
    10981 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
    11041 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
    11101 aggtcctcgc catggatcct gatgatgttg ttattcttct aatcttttgt atggaaaact
    11161 tttcttcgta ccacgggact aaacctggtt atgtagattc cattcaaaaa ggtatacaaa
    11221 agccaaaatc tggtacacaa ggaaattatg acgatgattg gaaagggttt tatagtaccg
    11281 acaataaata cgacgctgcg ggatactctg tagataatga aaacccgctc tctggaaaag
    11341 ctggaggcgt ggtcaaagtg acgtatccag gactgacgaa ggttctcgca ctaaaagtgg
    11401 ataatgccga aactattaag aaagagttag gtttaagtct cactgaaccg ttgatggagc
    11461 aagtcggaac ggaagagttt atcaaaaggt tcggtgatgg tgcttcgcgt gtagtgctca
    11521 gccttccctt cgctgagggg agttctagcg ttgaatatat taataactgg gaacaggcga
    11581 aagcgttaag cgtagaactt gagattaatt ttgaaacccg tggaaaacgt ggccaagatg
    11641 cgatgtatga gtatatggct caagcctgtg caggaaatcg tgtcaggcga tctctttgtg
    11701 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
    11761 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
    11821 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgcagat
    11881 cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    11941 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    12001 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    12061 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    12121 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgacctcgag ggggggcccg
    12181 cggccgccct gcagggcgat cgctacgtac agcttttgtt ccctttagtg agggttaatt
    12241 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    12301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    12361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    12421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    12481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    12541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    12601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    12661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    12721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    12781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    12841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    12901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    12961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    13021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    13081 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    13141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    13201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    13261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    13321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    13381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    13441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    13501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    13561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    13621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    13681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    13741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    13801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    13861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    13921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    13981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    14041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    14101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    14161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    14221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    14281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    14341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    14401 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
    14461 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
    14521 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
    14581 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
    14641 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
    14701 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
    14761 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
    14821 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
    14881 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    14941 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    15001 cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
    15061 tggagct
//
