LOCUS       sequence-127282-        2507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  artificial transcription template ""distal pause"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J,
            Gelles J
  TITLE     Bacterial RNA polymerase can retain sigma70 throughout 
            transcription.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 5. pii: 201513899.
  PUBMED    26733675
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 5. pii: 201513899."
COMMENT     Sequence Label: pTH07
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..27
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2421..2438)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2421..2437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2430..2452)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattccag acaacatttt
       61 gaatgcggtc acacgttagc agcatgattg ccacggatgg caacatatta acggcatgat
      121 attgacttat tgaataaaat tgggtaaatt tgactcaacg atgggttaat tcgctcggat
      181 ccagacacca cagaccacac acaagacacc acagaccaca cacaagacac cacagaccac
      241 acacaagaca ccacagacca cacacaagac accacagacc acacacaaga caccacagac
      301 cacacacaag acaccacaga ccacacacag catgcaacct gttcgtacgt atcgaccgtc
      361 gtgctataat gggttaattc gctccgtaaa ctgcctgcga ccatcattct gcgcgccctg
      421 aactacacca cagagcagat cctcgacctg ttctttgaaa aagttatctt tgaaatccgt
      481 gataacaagc tgcagatgga actggtgccg gaacgcctgc gtggtgaaac cgcatctttt
      541 gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc gccgtatcac tgcgcgccac
      601 attcgccagc tggaaaaaga cgacgtcaaa ctgatcgaag tcccggttga gtacatcgca
      661 ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg gcgagctgat ctgcgcagcg
      721 aacatggagc tgagcctgga tctgctggct aagctgagcc agtctggtca caagcgtatc
      781 gaaacgctgt tcaccaacga tctggatcac ggcccatata tctctgaaac cttacgtgtc
      841 gacccaacta acgaccgtct gagcgcactg gtagaaatct accgcatgat gcgccctggc
      901 gagccgccga ctcgtgaagc agctgaaagc ctgttcgaga acctgttctt ctccgaagac
      961 cgttatgact tgtctgcggt tggtcgtatg aagttcaacc gttctctgct gcgcgaagaa
     1021 atcgaaggtt ccggtatcct gagcaaagac gacatcattg atgttatgaa aaagctcatc
     1081 gatatccgta acggtaaagg cgaagtcgat gatatcgacc acctcggcaa ccgtcgtatc
     1141 cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg gcctggtacg tgtagagcgt
     1201 gcggtgaaag agcgtctgtc tctgggcgat ctggataccc tgatgccaca ggatatgatc
     1261 aacgccaagc cgatttccgc agcagtgaaa gagttcttcg gttccagcca gctgtctcag
     1321 tttatggacc agaacaaccc gctgtctgag attacgcaca aacgtcgtat ctccgcactc
     1381 ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag ttcgagacgt acacccgact
     1441 cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc cgaacatcgg tctgatcaac
     1501 tctctgtccg tgtacgcaca gactaacgaa tacggcttcc ttgagactcc gtatcgtaaa
     1561 gtgaccgacg gtgttgtaac tgacgaaatt cactacctgt ctgctatcga agaaggcaac
     1621 tacgttatcg cccaggcgaa ctccaacttg gatgaagaag gccacttcgt agaagacctg
     1681 gtaacttgcc gtagcaaagg cgaatccagc ttgttcagcc gcgaccaggt tgactacatg
     1741 gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc tgatcccgtt cctggaacac
     1801 gatgacgcca accgtgcatt gatgggtgcg aacatgcaac gtcaggccgt tccgactctg
     1861 cgcgctgata agccgctggt tggtactggt atggaacgtg ctgttgccgt tgactccggt
     1921 gtaactgcgg tagctaaacg tggtggtgtc gttcagtacg tggatgcttc ccgtatcgtt
     1981 atcaaagtta acgaagacga gatgtatccg ggtgaagcag gtatcgacat ctacaacctg
     2041 accaaataca cccgttctaa ccagaacacc tgtatcaacc agatgccgtg tgtgtctctg
     2101 ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc cgtccaccga cctcggtgaa
     2161 ctggcgcttg gtcagaacat cgcttactac cgattccgcc tagttggtca cttcgacgta
     2221 tcgtctggaa ctccaaccat cgcaggcaga gaggtctgca aaatgcaatc ccgaaacagt
     2281 tcgcaggtaa tagttagagc ctgcataacg gtttcgggat tttttatatc tgcacaacag
     2341 gtaagagcat tgagtcgata atcgacacag gaaacacata tgaccatgat tacgaatttc
     2401 gacctgcagc caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2461 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccag
//
