LOCUS       sequence-127285-         270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to
            the Ad4 E4orf6 gene via P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6
FEATURES             Location/Qualifiers
     source          1..270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcgcagcagc cgctgccttc ggcgttctgt cagggagctt gacaggggat ctgggtccag
       61 gctctcccgt ctcatgatgc caattgctcg cagcatcagc cttcgggttc gtcgtgcaca
      121 gcatctgatc ctaatctcgc tcagatctga acaataggta cagcacagga ccaccagatt
      181 gttcagcagg ccgtaattca gagtagacca cccaaaggac atctggggga caatggagcc
      241 cacatgcccg tcataccaga tccgaatgta
//
