LOCUS       sequence-127289-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Direct conversion of fibroblasts to functional neurons by defined 
            factors.
  JOURNAL   Nature. 2010 Feb 25. 463(7284):1035-41.
  PUBMED    20107439
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Feb 25. 463(7284):1035-41."
COMMENT     Sequence Label: Tet-O-FUW-Neurod1
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    182..200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    266..284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    308..326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        359..397
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     394..418
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
       61 gcagagatcc agtttggtta gatctcgagt ttaccactcc ctattagtga tagagaaaag
      121 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
      181 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      241 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      301 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg
      361 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
      421 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
      481 ccgcggcccc gaattcgcta gccaccatga ccaaatcata cagcgagagc gggctgatgg
      541 gcgagcctca gccccaaggt cccccaagct ggacagatga gtgtctcagt tctcaggacg
      601 aggaacacga ggcagacaag aaagaggacg agcttgaagc catgaatgca gaggaggact
      661 ctctgagaaa cgggggagag gaggaggagg aagatgagga tctagaggaa gaggaggaag
      721 aagaagagga ggaggaggat caaaagccca agagacgggg tcccaaaaag aaaaagatga
      781 ccaaggcgcg cctagaacgt tttaaattaa ggcgcatgaa ggccaacgcc cgcgagcgga
      841 accgcatgca cgggctgaac gcggcgctgg acaacctgcg ca
//
