LOCUS       sequence-127298-         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on 
            CAG expression vector. DDdCas9VP192 protein is stabilised by 
            Trimethoprim..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: CAG-DDdCas9VP192-T2A-EGFP-ires-puro
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..495
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             520..546
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             550..570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaattgggat ccgccaccat gatcagtctg attgcggcgt tagcggtaga ttacgttatc
       61 ggcatggaaa acgccatgcc gtggaacctg cctgccgatc tcgcctggtt taaacgcaac
      121 accttaaata aacccgtgat tatgggccgc catacctggg aatcaatcgg tcgtccgttg
      181 ccaggacgca aaaatattat cctcagcagt caaccgagta cggacgatcg cgtaacgtgg
      241 gtgaagtcgg tggatgaagc catcgcggcg tgtggtgacg taccagaaat catggtgatt
      301 ggcggcggtc gcgttattga acagttcttg ccaaaagcgc aaaaactgta tctgacgcat
      361 atcgacgcag aagtggaagg cgacacccat ttcccggatt acgagccgga tgactgggaa
      421 tcggtattca gcgaattcca cgatgctgat gcgcagaact ctcacagcta ttgctttgag
      481 attctggagc ggcgaggctc cggaaccggt gccaccatgt acccatacga tgttccagat
      541 tacgcttcgc cgaagaaaaa gcgcaaggtc gaagcgtccg acaagaagta cagcatcggc
      601 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caagg
//
