LOCUS       sequence-127299-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on 
            CAG expression vector. DDdCas9VP192 protein is stabilised by 
            Trimethoprim..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: CAG-DDdCas9VP192-T2A-EGFP-ires-puro
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt tcacactctt
       61 cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat aggccacggt
      121 ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg cgatcagctt
      181 atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct gcacctcggt
      241 ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg caaaatcccg
      301 gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag gccgcttccg
      361 gatctcgccg ttggccaggg taatctcggt cttgaaaaag ttcatgatgt tgctgtagaa
      421 gaagtacttg gcggtagcct tgccgatttc ctgctcgctc ttggcgatca tcttccgcac
      481 gtcgtacacc ttgtagtcgc cgtacacgaa ctcgctttcc agcttagggt actttttgat
      541 cagggcggtt cccacgacgg cgttcaggta ggcgtcgtgg gcgtggtggt agttgttgat
      601 ctcgcgcact ttgtaaaact ggaaatcctt ccggaaatcg gacaccagct tggacttcag
      661 ggtgatcact ttcacttccc ggatcagctt gtcattctcg tcgtacttag tgttcatccg
      721 gga
//
