LOCUS       sequence-127306-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episomal plasmid encoding  dCas9VP192 and p53 shRNA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: pCXLE-dCas9VP192-T2A-EGFP-shP53
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..70
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             107..217
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             269..379
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             431..541
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             593..703
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacagcc ccaagaagaa
       61 gagaaaggtg gaggccagcc tcgagtgcga aagcggcggg ccggccgacg cccttgacga
      121 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
      181 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtcactac ccagcgctag
      241 cgtcgagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct
      301 cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga
      361 ttttgacctt gacatgctcc ccgggtcact acccagcgct agcgtcgagt gcgaaagcgg
      421 cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
      481 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
      541 ccccgggtca ctacccagcg ctagcgtcga gtgcgaaagc ggcgggccgg ccgacgccct
      601 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
      661 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt cactacccag
      721 cgctagcgtc
//
