LOCUS       sequence-127315-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Jones SA, Shim SH, He J, Zhuang X
  TITLE     Fast, three-dimensional super-resolution imaging of live cells.
  JOURNAL   Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8.
  PUBMED    21552254
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-06"; volume: "8(6)"; pages: "499-508. Epub 
            2011 May 8"
COMMENT     Sequence Label: ptdEos-CLC
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..725
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcaccatg gtggcgatta
       61 agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
      121 tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca
      181 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
      241 acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta
      301 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa
      361 acgacataac aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact
      421 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
      481 aaatgtatgt gcgtgatgga gtgctgaccg gtgatattcg catggctttg ttgcttgaag
      541 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
      601 agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt
      661 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
      721 gacgatctcg aggtcacggt actggttcta ctggttctgg ttcttctgag agtgcgatta
      781 agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
      841 tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca
      901 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
      961 acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta
     1021 aggggtattc gtgggaacga agcttgactt tcgaagacgg ggg
//
