LOCUS       sequence-127318-        6087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  improved luciferase reporter gene plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Bert AG, Burrows J, Osborne CS, Cockerill PN
  TITLE     Generation of an improved luciferase reporter gene plasmid that 
            employs a novel mechanism for high-copy replication.
  JOURNAL   Plasmid. 2000 Sep;44(2):173-82.
  PUBMED    10964627
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "173-82"
COMMENT     Sequence Label: pXPG
FEATURES             Location/Qualifiers
     source          1..6087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      70..79
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             76..1728
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(142..160)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          1813..1878
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2008..2028
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2450..2584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3355..3373)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3441..3545
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3546..3614,3615..4406)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3764..3783)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4577..5165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5066..5085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5319..5336
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5351..5491
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5417..5436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5577..5599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    5730..5864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    join(5958..6087,1..5)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatccaagc tcagatctcg agctcggtac ccgggtcgac aagcttggca ttccggtact
       61 gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
      121 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
      181 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
      241 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
      301 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
      361 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
      421 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
      481 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
      541 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
      601 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
      661 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
      721 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
      781 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
      841 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
      901 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
      961 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     1021 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     1081 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     1141 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     1201 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     1261 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     1321 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     1381 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     1441 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     1501 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     1561 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     1621 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     1681 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt ctagaggatc
     1741 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     1801 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     1861 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     1921 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     1981 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     2041 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     2101 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     2161 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     2221 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     2281 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     2341 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2401 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgtta acttgtttat
     2461 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2521 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2581 gatcgatccc caggaagctc ctctgtgtcc tcataaaccc taacctcctc tacttgagag
     2641 gacattccaa tcataggctg cccatccacc ctctgtgtcc tcctgttaat taggtcactt
     2701 aacaaaaagg aaattgggta ggggtttttc acagaccgct ttctaagggt aattttaaaa
     2761 tatctgggaa gtcccttcca ctgctgtgtt ccagaagtgt tggtaaacag cccacaaatg
     2821 tcaacagcag aaacatacaa gctgtcagct ttgcacaagg gcccaacacc ctgctcatca
     2881 agaagcactg tggttgctgt gttagtaatg tgcaaaacag gaggcacatt ttccccacct
     2941 gtgtaggttc caaaatatct agtgttttca tttttacttg gatcaggaac ccagcactcc
     3001 actggataag cattatcctt atccaaaaca gccttgtggt cagtgttcat ctgctgactg
     3061 tcaactgtag cattttttgg ggttacagtt tgagcaggat atttggtcct gtagtttgct
     3121 aacacaccct gcagctccaa aggttcccca ccaacagcaa aaaaatgaaa atttgaccct
     3181 tgaatgggtt ttccagcacc attttcatga gttttttgtg tccctgaatg caagtttaac
     3241 atagcagtta ccccaataac ctcagtttta acagtaacag cttcccacat caaaatattt
     3301 ccacaggtta agtcctcatt taaattaggc aaaggaattc ttgaagacga aagggcctcg
     3361 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     3421 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3481 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3541 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3601 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3661 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3721 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3781 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3841 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3901 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3961 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4021 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4081 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4141 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4201 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4261 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4321 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4381 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4441 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4501 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4561 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatatgctgc ttgcaaacaa
     4621 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4681 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4741 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4801 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4861 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4921 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4981 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5041 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5101 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5161 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5221 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5281 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5341 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5401 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     5461 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     5521 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5581 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc aggatcagcc
     5641 atatcacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     5701 tgaaacataa aatgaatgca attgttgtta acttgtttat tgcagcttat aatggttaca
     5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcagccat atcacatttg
     5881 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5941 tgaatgcaat tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6061 aactcatcaa tgtatcttat catgtct
//
