LOCUS       sequence-127322-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with an improved l scaffold and 
            puro/BFP markers without WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..115
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccggcggcg ggtccggagg
       61 agagggcaga ggaagtctcc taacatgcgg tgacgtggag gagaatcctg gcccaatgag
      121 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
      181 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
      241 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
      301 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
      361 gtccttccct gagggcttca catgggagag agtcaccaca tacgaggacg ggggcgtgct
      421 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
      481 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
      541 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
      601 gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa
      661 acccgctaag aacctcaaga tgcctggcgt ctac
//
