LOCUS       sequence-127326-        6058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to 
            generate RIP-OVAhi and RIP-OVA lo mice).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Blanas E, Carbone FR, Allison J, Miller JF, Heath WR
  TITLE     Induction of autoimmune diabetes by oral administration of 
            autoantigen.
  JOURNAL   Science. 1996 Dec 6;274(5293):1707-9.
  PUBMED    8939860
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1996-12-6"; volume: "274(5293)"; pages: "1707-9"
COMMENT     Sequence Label: pBlueRIP.OVA
FEATURES             Location/Qualifiers
     source          1..6058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     672..688
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     672..688
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    685..819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(709..728)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     763..782
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     intron          979..1044
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1832..2992)
                     /codon_start=1
                     /product="chicken ovalbumin"
                     /label=ovalbumin
                     /translation="MGSIGAASMEFCFDVFKELKVHHANENIFYCPIAIMSALAMVYLG
                     AKDSTRTQINKVVRFDKLPGFGDSIEAQCGTSVNVHSSLRDILNQITKPNDVYSFSLAS
                     RLYAEERYPILPEYLQCVKELYRGGLEPINFQTAADQARELINSWVESQTNGIIRNVLQ
                     PSSVDSQTAMVLVNAIVFKGLWEKTFKDEDTQAMPFRVTEQESKPVQMMYQIGLFRVAS
                     MASEKMKILELPFASGTMSMLVLLPDEVSGLEQLESIINFEKLTEWTSSNVMEERKIKV
                     YLPRMKMEEKYNLTSVLMAMGITDVFSSSANLSGISSAESLKISQAVHAAHAEINEAGR
                     EVVGSAEAGVDAASVSEEFRADHPFLFCIKHIATNAVLFFGRCVSP"
     misc_feature    3018..3074
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3821..3837)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3822..3838)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(3866..3884)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3905..3921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3905..3921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3918..3940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3929..3945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3953..3959,3960..3977,3978..3983))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4136..4153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4307..4895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5066..5857,5858..5926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5689..5708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5927..6031)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctccaccgcg
      661 gtggcggccg ctctagaact agtggatcca gacatgataa gatacattga tgagtttgga
      721 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
      781 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
      841 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
      901 aaatgtggta tggctgatta tgatcatgaa cagactgtga ggactgaggg gcctgaaatg
      961 agccttggga ctgtgaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1021 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1081 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc ccaagcttgg atctggggcg
     1141 cccatgggcg aattcctttt tttttttttt tttttttttt ttttgcttaa acatgttttt
     1201 attatgatta aaggaacaaa agagcacatt cacaagaccc attacatatg ggtacaagga
     1261 aaacaatttg aatagtaata taccatattt gccaacatac catgattgag tcaaagttta
     1321 gggagaaatg tgaattataa gatttttata atgcatcttt aggaagtcag gaagagcctt
     1381 gtagtatcag gaacacagag aacaagcaat tgccttgtca gcataggaat ggttggtgac
     1441 agttgataat ttaatctgag agattttgag tgactaattc tggagcagct tggtcataca
     1501 gatatctggc ttaattggaa ggctgcattt ttcccccata aaccttctgc tgatgtatca
     1561 ggttgcattt ttcagtgtga tgactcagta ctgtgagtcc aatttcattc ccttaagcct
     1621 tcatccatga gttaccagta ttactctgtg taaaggaaaa gtgaattgca cctgttctca
     1681 cagtgtaatt tctttctgat tttttttcta gattaagctc cagcttttat gaagtctgga
     1741 tgcagcagat aacatacttt tcattttacc cctgatacta cagtgctctg ggtcttgttg
     1801 gaagggacag agtttttcag ctttcttctt tttaagggga aacacatctg ccaaagaaga
     1861 gaacggcgtt ggttgcgatg tgcttgatac agaagaggaa tggatggtca gccctaaatt
     1921 cttcagagac gcttgcagca tccactccag cctctgctga ccctaccacc tctctgcctg
     1981 cttcattgat ttctgcatgt gctgcatgga cagcttgaga tatcttcagg ctctctgctg
     2041 aggagatgcc agacagattg gctgaagagc taaacacgtc agtaatgccc atagccatta
     2101 agacagatgt gaggttgtat ttttcctcca tcttcatgcg aggtaagtac actttgatct
     2161 tcctctcttc cataacatta gaactggtcc attcagtcag tttttcaaag ttgattatac
     2221 tctcaagctg ctcaaggcct gagacttcat caggcaacag caccaacatg ctcattgtcc
     2281 cactggcaaa tggaagctcc aggatcttca ttttctcaga agccattgat gccactctaa
     2341 ataaaccaat ctggtacatc atctgcacag gtttgctttc ttgctcagtc actctgaaag
     2401 gcattgcttg tgtgtcttca tccttaaatg ttttctccca cagtcctttg aagacaatgg
     2461 cattaaccag aaccattgca gtttgagaat ccacggagct tggctgaagg acatttctga
     2521 taattccatt tgtctgactt tctacccagg aattgatgag ctctctggct tgatctgcag
     2581 ctgtttgaaa gttgataggt tccaagcctc ctctatacag ttccttcaca cactgcaagt
     2641 attctggcag gattgggtat ctctcttcag cataaagtct actggcaagg ctgaacgaat
     2701 aaacatcatt tggtttggtg atttggttga ggatgtctct aagtgaagag tgaacgttta
     2761 cagatgtgcc acactgagct tcaatactgt ctccgaatcc tggaagttta tcaaagcgaa
     2821 caaccttatt tatttgtgtc ctggtgctgt cttttgcacc caggtatacc atggctagag
     2881 ctgacatgat ggcaatgggg cagtagaaga tgttctcatt ggcatggtgg actttgagct
     2941 ccttgaatac atcaaaacaa aattccatgc ttgctgcacc gatggagccc atggtgaact
     3001 ctgagttgtc tttcgaggaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
     3061 aggcatgcaa gcttgctaga gttagggctg ggggttactg aatccccact agctttatag
     3121 tccaaagcac cccccccctg acccctggac tttgctgttt gtcccattaa gggtggatca
     3181 gcagatggcc agaggggctg aagctgcagt ttccaaacac ttgcctggtg ctaggtctgc
     3241 agaaagtgct cattggacaa caacacctct acttagcctt agggtaatta gagtcttaat
     3301 aggggctcct gggtggcctg atgaaccagt tcacagcagg ggacattgtc tcagagaggg
     3361 tgaggccttc tttgtccttg gctgagcatt tttccacatc attccccagg aggctgcagt
     3421 tgttggggga cctagtaact ttgtcccttg aactgttgtt tcacagttcc cactgatagc
     3481 tgtgtcctca cctcagctga gaccccagct caccctcaga ggtagaagga aagcagaatt
     3541 caagaagaaa ggtcccttct cttctatctc tccttccaca aacccatagc ccactcggga
     3601 gtcaactggg ctgaagcccc atggtgtatt ctcctgtagt atcttagatt tgcacctgaa
     3661 ggtcagcaac tctccataag aggtgcttgc ttcagagctc agagccagct gtctccatct
     3721 cttagtacat actctaccca gctacaaaac ctttctcagc ctccacttgg agtggttggg
     3781 ggatcccccg ggctgcagga attcgatatc aagctcttat cgataccgtc gacctcgagg
     3841 gggggcccgg taccagcttt tgttcccttt agtgagggtt aattccgagc ttggcgtaat
     3901 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatag
     3961 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa
     4021 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4081 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4141 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4201 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4261 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcg
     4321 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4381 gactataaag ataccaggcg ttcccccctg gaagctccct cgtgcgctct cctgttccga
     4441 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4501 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4561 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4621 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4681 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4741 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4801 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4861 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4921 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4981 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5041 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5101 cgatctgtct atttcgttca tccatagttg cctgactgcc cgtcgtgtag ataactacga
     5161 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5221 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5281 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5341 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5401 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5461 gatcccccat gttgtgaaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5521 gtaagttggc cgcagtgtta tcactcatgc ttatggcagc actgcataat tctcttactg
     5581 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5641 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5701 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5761 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5821 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5881 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5941 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6001 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctga
//
