LOCUS       sequence-127327-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP11-clathrin light chain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pEGFP-GFP11-Clathrin light chain
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..51
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 atgcgtgacc acatggtcct tcatgagtat gtaaatgctg ctgggattac aggcggatcc
       61 ggactcagat ctcgagctca agcttcgaat tctgcagtcg acatggctga tgactttggc
      121 ttcttctcgt cgtcggagag tggtgccccg gaggcggcgg aggaggaccc ggcggccgcc
      181 ttcctggccc agcaggagag cgagattgca ggcatagaga acgacgaggg cttcggggca
      241 cctgccggca gccatgcggc ccccgcacag ccgggcccca cgagtggggc tggttctgag
      301 gacatgggga ccacagtcaa tggagatgtg tttcaggagg ccaacggtcc tgctgatggc
      361 tacgcagcca ttgcccaggc tgacaggctg acccaggagc ctgagagcat ccgcaagtgg
      421 cgagaggagc agaggaaacg gctgcaagag ctggatgctg catctaaggt cacggaacag
      481 gaatggcggg agaaggccaa gaaggacctg gaggagtgga accagcgcca gagtgaacaa
      541 gtagagaaga acaagatcaa caaccgggca tccgaggagg ctttcgtgaa ggaatccaag
      601 gaggagaccc caggcacaga gtgggagaag gtggcccagc tatgtgactt caaccccaag
      661 agcagcaagc agtgcaaaga tgtgtcccgc ctgcgctcgg tgctcatgtc cctgaagcag
      721 acgccactgt cccgctag
//
