LOCUS       sequence-127330-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP37
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             70..92
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             133..165
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(169..177,178..192)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(208..210,211..723)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(250..271)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(511..530)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     858..879
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttctctctt ttatacacac attcaaaaga aagaaaaaaa atatacccca gcctcgatct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      181 gatgacgata aggatccggg cctcgaggtg agcaagggcg aggagctgtt caccggggtg
      241 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      301 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      361 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gatgtgcttc
      421 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      481 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      541 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      601 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      661 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      721 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      781 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
      841 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatca
//
