LOCUS       sequence-127337-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the human codon optimized CDS of Cas9 protein as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: phumanCas9 (GB0575)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(88..108)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acacgcgttg ggagctctcc catatggtcg acctgcaggc ggccgcgaat tcactagtga
       61 ttgggcgatg agtggtctcg aagctcacac cttcctcttc ttcttggggt cagccctgct
      121 gtctccaccg agctgagaga ggtcgattct tgtttcatag agccccgtaa ttgactgatg
      181 aatcagtgtg gcgtccagga cctcctttgt agaggtgtac cgctttctgt ctatggtggt
      241 gtcgaagtac ttgaaggctg caggcgcgcc caagttggtc agagtaaaca agtggataat
      301 gttttctgcc tgctccctga tgggcttatc cctgtgctta ttgtaagcag aaagcacctt
      361 atcgaggtta gcgtcggcga ggatcactct tttggagaat tcgcttattt gctcgatgat
      421 ctcatcaagg tagtgtttgt gttgttccac gaacagctgc ttctgctcat tatcttcggg
      481 agatcctttg agcttttcat agtggctggc cagatacaag aaattaacgt atttagaggg
      541 cagtgccagc tcgttacctt tctgcagctc gcccgcacta gcgagcattc gtttccggcc
      601 gttttcaagc tcaaagagag agtacttggg aagcttaatg atgaggtctt ttttgacctc
      661 tttatatcct ttcgcctcga gaaagtcgat ggggtttttt tcgaagcttg atcgctccat
      721 gattgtgatg cccagcagtt ccttgacgct tttgagtttt ttagacttcc ctttctccac
      781 tttggccaca accagtacac tgtaagcgac tgtaggagaa tcgaatccgc cgtatttctt
      841 ggggtcccaa tc
//
