LOCUS       sequence-127347-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the human codon optimized CDS of Cas9 protein with mutated 
            (D10A, H840A) and inactivated catalytic domains as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pD10AH840AhCas9 (GB1041)
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(89..109)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa ttcactagtg
       61 attgggcgat gagtggtctc gaagctcaca ccttcctctt cttcttgggg tcagccctgc
      121 tgtctccacc gagctgagag aggtcgattc ttgtttcata gagccccgta attgactgat
      181 gaatcagtgt ggcgtccagg acctcctttg tagaggtgta ccgctttctg tctatggtgg
      241 tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac aagtggataa
      301 tgttttctgc ctgctccctg atgggcttat ccctgtgctt attgtaagca gaaagcacct
      361 tatcgaggtt agcgtcggcg aggatcactc ttttggagaa ttcgcttatt tgctcgatga
      421 tctcatcaag gtagtgtttg tgttgttcca cgaacagctg cttctgctca ttatcttcgg
      481 gagatccttt gagcttttca tagtggctgg ccagatacaa gaaattaacg tatttagagg
      541 gcagtgccag ctcgttacct ttctgcagct cgcccgcact agcgagcatt cgtttccggc
      601 cgttttcaag ctcaaagaga gagtacttgg gaagcttaat gatgaggtct tttttgacct
      661 ctttatatcc tttcgcctcg agaaagtcga tggggttttt ttcgaagctt gatcgctcca
      721 tgattgtgat gcccagcagt tccttgacgc ttttgagttt tttagacttc cctttctcca
      781 ctttggccac aaccagtaca ctgtaagcga ctgtaggaga atcgaatccg ccgtatttct
      841 tggggtccca atcttttttg cgtgcgatca gcttgtcgct gttccttttc gggaggatac
      901 tttccttgga
//
