LOCUS       sequence-127349-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the human codon optimized CDS of Cas9 protein with mutated 
            (D10A, H840A) and inactivated catalytic domains as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pD10AH840AhCas9 (GB1041)
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgaatggaca
       61 agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg
      121 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      181 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggaaacggcc gaagccacgc
      241 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      301 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      361 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      421 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
      481 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
      541 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
      601 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
      661 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
      721 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
      781 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
      841 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
      901 gcgaccagta cgc
//
