LOCUS       sequence-127356-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pU6-26 (GB1001)
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      324..333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      328..337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(367..386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(368..386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(393..410)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(393..409)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(402..424)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(637..656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcacgcgttg ggagctctcc catatggtcg acctgcaggc ggccgcgaat tcactagtga
       61 ttgggcgatg agtggtctcg ggagcatctt cattcttaag atatgaagat aatcttcaaa
      121 aggcccctgg gaatctgaaa gaagagaagc aggcccattt atatgggaaa gaacaatagt
      181 atttcttata taggcccatt taagttgaaa acaatcttca aaagtcccac atcgcttaga
      241 taagaaaacg aagctgagtt tatatacagc tagagtcgaa gtagtgattg cgagaccact
      301 catcgcccaa tcgaattccc gcggccgcca tggcggccgg gagcatgcga cgtcgggccc
      361 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
      421 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      481 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      541 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      601 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      721 tccgatttag tgctttacgg cacctcgacc cc
//
