LOCUS       sequence-127363-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mPAGFP-mRuby2-FRET-10
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(131..382)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSHQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(173..194)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     522..541
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
      121 ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      181 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtggc
      241 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      301 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttggcct
      361 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      421 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      481 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      541 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      601 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag ctgaaggtgg
      661 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      721 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      781 cgtcgccgtc cagctcgacc
//
