LOCUS       sequence-127365-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  detection/assessment of OCT4 and SOX2 transcription factor activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     luciferase reporter plasmid containing 6 tandem copies of the 
            composite Oct4/Sox2 binding site of the FGF4 enhancer. Used for 
            detection of active Oct4/Sox2 complexes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 6xO/S luc
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    314..435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(351..370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     405..424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     498..514
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     498..514
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      181 tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt acgtggaggt
      241 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      301 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      361 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      421 catcaatgta tcttatggta ctgtaactga gctaacataa cccgggaggt accgagctcc
      481 accgcggtgg cggccgctct agaactagtg gatctgaaaa ctctttgttt ggatgctaat
      541 gggatactta aaggatctga aaactctttg tttggatgct aatgggatac ttaaaggatc
      601 tgaaaactct ttgtttggat gctaatggga tacttaaagg atctgaaaac tctttgtttg
      661 gatgctaatg ggatacttaa aggatctgaa aactctttgt ttggatgcta atgggatact
      721 taaaggatct gaaaactctt tgtttggatg ctaatgggat acttaaagga tcccccgggc
      781 gcggggcagg cgagtaggag ggggcgccgg ctatatatac cactgctccg gagggctggg
      841 cgcgcgggga ctatcccgcc accgttgcgt ccctatttgc tctcgctact taggtctgtg
      901 cgcagcactc a
//
